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ITIH2 antibody

Anti-ITIH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04998
Anti-ITIH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04998
Anti-ITIH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04998
Anti-ITIH2 antibody used in Western Blot (WB). GTX04998
Anti-ITIH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04998

Cat. No. GTX04998

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all ITIH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Dog, Chicken, Pig, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50%Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ITIH2(Accession P19823), corresponding to amino acid residues Q901-P946.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2P,ITIH2,SHAP,interalphatrypsin inhibitor heavy chain 2,inter-alpha-trypsin inhibitor heavy chain 2

Cellular Localization

Secreted

Background

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-ITIH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04998

GTX04998 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04998 ITIH2 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-ITIH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04998

GTX04998 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04998 ITIH2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-ITIH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04998

GTX04998 IHC-P Image

IHC-P analysis of human kidney cancer and adjacent normal tissue using GTX04998 ITIH2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-ITIH2 antibody used in Western Blot (WB). GTX04998

GTX04998 WB Image

WB analysis of B16F10 whole cell lysate using GTX04998 ITIH2 antibody. The lane on the left was treated with blocking peptide.

Anti-ITIH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04998

GTX04998 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04998 ITIH2 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 419