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ITIH3 antibody [N1N3]

Anti-ITIH3 antibody [N1N3] used in Western Blot (WB). GTX112448
Anti-ITIH3 antibody [N1N3] used in Western Blot (WB). GTX112448
Anti-ITIH3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112448
Anti-ITIH3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112448

Cat. No. GTX112448

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ITIH3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

BCL-1 , A431

Predict Reactivity

Rat, Rabbit, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITIH3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037280

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inter-alpha-trypsin inhibitor heavy chain 3 , H3P , ITI-HC3 , SHAP

Cellular Localization

Secreted

Background

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ITIH3 antibody [N1N3] used in Western Blot (WB). GTX112448

GTX112448 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX112448 diluted at 1:1000

Anti-ITIH3 antibody [N1N3] used in Western Blot (WB). GTX112448

GTX112448 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
7.5% SDS PAGE
GTX112448 diluted at 1:1000

Anti-ITIH3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112448

GTX112448 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ITI-H3(GTX112448) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ITIH3 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX112448

GTX112448 IHC-P Image

ITI-H3 antibody [N1N3] detects ITI-H3 protein at secreted on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ITI-H3 antibody [N1N3] (GTX112448) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169