Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [HL1264] (GTX636666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ITK antibody [HL1264] detects ITK protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
ITK stained by ITK antibody [HL1264] (GTX636666) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ITK antibody [HL1264] detects ITK protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ITK stained by ITK antibody [HL1264] (GTX636666) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

ITK antibody [HL1264] detects ITK protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ITK stained by ITK antibody [HL1264] (GTX636666) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min