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ITK antibody [N2C1], Internal

Orthogonal
Anti-ITK antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113217
Anti-ITK antibody [N2C1], Internal used in Western Blot (WB). GTX113217
Anti-ITK antibody [N2C1], Internal used in Western Blot (WB). GTX113217

Cat. No. GTX113217

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ITK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037282

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IL2 inducible T cell kinase , EMT , LPFS1 , LYK , PSCTK2

Cellular Localization

Cell membrane

Background

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ITK antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113217

GTX113217 ICC/IF Image

ITK antibody [N2C1], Internal detects ITK protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ITK stained by ITK antibody [N2C1], Internal (GTX113217) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ITK antibody [N2C1], Internal used in Western Blot (WB). GTX113217

GTX113217 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (GTX113217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITK antibody [N2C1], Internal used in Western Blot (WB). GTX113217

GTX113217 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (GTX113217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169