Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) unboiled Capan-2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

ITM2B antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
ITM2B antibody (GTX119361) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using ITM2B(GTX119361) antibody at 1:200 dilution.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.