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ITM2B antibody

Anti-ITM2B antibody used in Western Blot (WB). GTX119361
Anti-ITM2B antibody used in Western Blot (WB). GTX119361
Anti-ITM2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119361
Anti-ITM2B antibody used in IHC (Paraffin sections) (IHC-P). GTX119361
Anti-ITM2B antibody used in Western Blot (WB). GTX119361

Cat. No. GTX119361

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

rat brain , RT-4

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITM2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620085

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integral membrane protein 2B , ABRI , BRI , BRI2 , BRICD2B , E25B , E3-16 , FBD , RDGCA , imBRI2

Cellular Localization

Golgi apparatus membrane; Single-pass type II membrane protein

Background

Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ITM2B antibody used in Western Blot (WB). GTX119361

GTX119361 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITM2B antibody used in Western Blot (WB). GTX119361

GTX119361 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ITM2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119361

GTX119361 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ITM2B(GTX119361) antibody at 1:200 dilution.

Anti-ITM2B antibody used in IHC (Paraffin sections) (IHC-P). GTX119361

GTX119361 IHC-P Image

ITM2B antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
ITM2B antibody (GTX119361) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ITM2B antibody used in Western Blot (WB). GTX119361

GTX119361 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (GTX119361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169