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ITM2C antibody

Anti-ITM2C antibody used in IHC (Paraffin sections) (IHC-P). GTX116904
Anti-ITM2C antibody used in Western Blot (WB). GTX116904

Cat No. GTX116904

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITM2C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624534

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integral membrane protein 2C , BRI3 , BRICD2C , E25 , E25C , ITM3

Cellular Localization

Membrane; Single-pass type II membrane protein

Background

Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.

Database

Research Area

DATA IMAGES

Anti-ITM2C antibody used in IHC (Paraffin sections) (IHC-P). GTX116904

GTX116904 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ITM2C(GTX116904) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ITM2C antibody used in Western Blot (WB). GTX116904

GTX116904 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
GTX116904 diluted at 1:1000

REFERENCE

REVIEW

ITM2C antibody Cat No. GTX116904

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : FACS
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31844045
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169