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IZUMO1 antibody

Anti-IZUMO1 antibody used in Western Blot (WB). GTX00746
Anti-IZUMO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00746
Anti-IZUMO1 antibody used in Western Blot (WB). GTX00746
Anti-IZUMO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00746

Cat No. GTX00746

Host

Rabbit

Clonality

Polyclonal

Application

WB, ICC/IF, IHC-P, Inhibition assay

Reactivity

Human, Mouse
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:100-1:300
IHC-P 1:100
Inhibition assay Assay dependent

Note :

Inhibition assay
It shows the inhibition of sperm fusion with egg.

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum with 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptides corresponding to the following three regions of human IZUMO1:
[A] C+KSLEKDYLPGHLDA
[B] C+TQVPKEKATDSRQQ
[C] C+ATTESSISLQPLQ

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

izumo sperm-egg fusion 1 , IZUMO , OBF

Cellular Localization

Cell membrane

Background

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-IZUMO1 antibody used in Western Blot (WB). GTX00746

GTX00746 WB Image

WB analysis of human sperm using GTX00746 IZUMO1 antibody.
Dilution : 1:1000
Loading : 20 μg

Anti-IZUMO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00746

GTX00746 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00746 IZUMO1 antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

Anti-IZUMO1 antibody used in Western Blot (WB). GTX00746

GTX00746 WB Image

WB analysis of mouse sperm using GTX00746 IZUMO1 antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel

Anti-IZUMO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00746

GTX00746 ICC/IF Image

ICC/IF analysis of human sperms using GTX00746 IZUMO1 antibody.
Lower : Primary antibody
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 359