Iba1 antibody [GT10312]

Cat No. GTX632426

Reference 4

Host	Mouse
Clonality	Monoclonal
Clone Name	GT10312
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat
Package	100 μl ($319), 25 μl ($169)

See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
FACS	1:50-1:200

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

allograft inflammatory factor 1,AIF-1,IBA1,IRT-1,IRT1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 199 AIF1
UniProt: P55008 AIF1

Research Area

DATA IMAGES

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at microglia in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632426 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at microglia in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632426 ICC/IF Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 protein stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632426 WB Image

Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632426 FACS Image

Iba1 antibody [GT10312] (GTX632426) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody [GT10312] (GTX632426) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

REFERENCE