Iba1 antibody [GT10312]

Name: Iba1 antibody [GT10312]
Brand: GeneTex
SKU: GTX632426
Price: 419.00 USD
Rating: 4.3 (3 reviews)

Cat. No. GTX632426

3 45

Cat. No. GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

1 / 9

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

2 / 9

GTX632426 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

3 / 9

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

4 / 9

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at microglia by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

5 / 9

GTX632426 FCM Image

Iba1 antibody [GT10312] (GTX632426) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody [GT10312] (GTX632426) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

6 / 9

GTX632426 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

7 / 9

GTX632426 Image

The data was published in the journal Front Mol Neurosci in 2019.PMID: 30914921

8 / 9

GTX632426 Image

The data was published in the journal Front Pharmacol in 2020.PMID: 32194421

9 / 9

This Iba1 antibody [GT10312] (GTX632426) is a mouse monoclonal antibody validated for detection of Iba1 by WB, IHC-P, FCM, and IHC (Free Floating). Reactive to human, mouse, rat, and other species. This product is cited in 45 published studies.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT10312
Isotype

IgG1
Applications

WB IHC-P FCM IHC (Free Floating)
Reactivity

Human, Mouse, Rat, Zebrafish