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Iba1 antibody [GT10312]

Orthogonal
Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426
Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426
Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426
Anti-Iba1 antibody [GT10312] used in Flow cytometry (FACS). GTX632426

Cat No. GTX632426

Reference 20
5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT10312

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888314

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426

GTX632426 ICC/IF Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 protein stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate

Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426

GTX632426 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426

GTX632426 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [GT10312] used in Flow cytometry (FACS). GTX632426

GTX632426 FACS Image

Iba1 antibody [GT10312] (GTX632426) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody [GT10312] (GTX632426) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

Application Reference

Fei X et al. J Neuroinflammation 2022; 19 (1) : 67 Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage.
Reactivity : Mouse
Chenyu Zhang et al. Redox Biol 2021; 47 : 102134 The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease.
Weng J et al. Theranostics 2021; 11 (16) : 7620-7639 Fiber scaffold bioartificial liver therapy relieves acute liver failure and extrahepatic organ injury in pigs.
Application : IHC-P
Reactivity : Human
Feng X et al. Brain Behav Immun 2021; 98 : 283-298 LncRNA 4344 promotes NLRP3-related neuroinflammation and cognitive impairment by targeting miR-138-5p.
Application : IHC-P
Reactivity : Rat
Ciervo Y et al. Mol Ther Methods Clin Dev 2021; 21 : 413-433 Adipose-derived stem cells protect motor neurons and reduce glial activation in both in?vitro and in?vivo models of ALS.
Application : IHC-P
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Iba1 antibody [GT10312] Cat No. GTX632426

Rating
( Average 4.6 based on 5 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.5 based on 2 users reviews)
IHC (Free Floating)(IHC (Free Floating))
( Average 4.5 based on 2 users reviews)
Anti-Iba1 antibody [GT10312]. GTX632426
Date : Anonymous submitted on 10-Aug-2021
Score :
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Genome Biol in 2019. PMID: 30791937
Anti-Iba1 antibody [GT10312] used in IHC (Free Floating) (IHC (Free Floating)). GTX632426
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29967381
Anti-Iba1 antibody [GT10312] used in IHC (Free Floating) (IHC (Free Floating)). GTX632426
Date : Anonymous submitted on 08-Apr-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Mouse P28 brain tissue
Amount used : 4%PFA
Blocking : TNB blocking buffer, 25°C, 1Hr
Permeabilization /Antigen retrieval : 0.01M citrate buffer, pH6.0
Primary Antibody : 1:400, 4°C, 16Hr
Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426
Date : Anonymous submitted on 08-Apr-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : BV2 cells
Blocking : TNB blocking buffer, 25°C, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.3% TritonX-100 in PBS
Primary Antibody : 1:400, 4°C, 16Hr
Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426
Date : Tanusree Sen submitted on 26-Dec-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : mouse microglia primary culture
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, 25°C, 0.5Hr
Primary Antibody : 1:1:100, 4°C, 12Hr
Submit a Review
Application Reference
Fei X et al. J Neuroinflammation 2022; 19 (1):67 Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage.
Reactivity : Mouse
Chenyu Zhang et al. Redox Biol 2021; 47:102134 The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease.
Weng J et al. Theranostics 2021; 11 (16):7620-7639 Fiber scaffold bioartificial liver therapy relieves acute liver failure and extrahepatic organ injury in pigs.
Application : IHC-P
Reactivity : Human
Feng X et al. Brain Behav Immun 2021; 98:283-298 LncRNA 4344 promotes NLRP3-related neuroinflammation and cognitive impairment by targeting miR-138-5p.
Application : IHC-P
Reactivity : Rat
Zhao M et al. Cell Death Differ 2021; The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.
Reactivity : Mouse
Ciervo Y et al. Mol Ther Methods Clin Dev 2021; 21:413-433 Adipose-derived stem cells protect motor neurons and reduce glial activation in both in?vitro and in?vivo models of ALS.
Application : IHC-P
Reactivity : Mouse
Jia Y et al. Tissue Cell 2021; 71:101514 Protective effects of oridonin against cerebral ischemia/reperfusion injury by inhibiting the NLRP3 inflammasome activation.
Liu D et al. Front Pharmacol 2020; 11:204 Purmorphamine Attenuates Neuro-Inflammation and Synaptic Impairments After Hypoxic-Ischemic Injury in Neonatal Mice via Shh Signaling.
Zhong Z et al. Neurobiol Dis 2020; 143:104945 Bone marrow mesenchymal stem cells upregulate PI3K/AKT pathway and down-regulate NF-κB pathway by secreting glial cell-derived neurotrophic factors to regulate microglial polarization and alleviate deafferentation pain in rats.
Application : ICC/IF
Reactivity : Rat
Zhong Z et al. Stem Cells Dev 2020; (Epub) Adipose-Derived Stem Cells Modulate BV2 Microglial M1/M2 Polarization by Producing Glial Cell-Derived Neurotrophic Factor.
Reactivity : Mouse
Liu C et al. Neuroscience 2020; 432:84-93 Activation of the Notch Signaling Pathway and Cellular Localization of Notch Signaling Molecules in the Spinal Cord of SOD1-G93A ALS Model Mice.
Reactivity : Mouse
Shraddha Sharma et al. Genome Biol 2019; 20 (1):37 Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.
Reactivity : Mouse
Zhou X et al. Front Mol Neurosci 2019; 12:58 L-Cysteine-Derived H2S Promotes Microglia M2 Polarization via Activation of the AMPK Pathway in Hypoxia-Ischemic Neonatal Mice.
Van Weehaeghe D et al. J Nucl Med 2019; TSPO versus P2X7 as target for neuroinflammation - an in vitro and in vivo study.
Application : IHC-P
Reactivity : Human
Zhou X et al. Biomed Pharmacother 2019; 115:108818 Mesenchymal stem cell derived EVs mediate neuroprotection after spinal cord injury in rats via the microRNA-21-5p/FasL gene axis.
Rosenzweig N et al. Nat Commun 2019; 10 (1):465 PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse model.
Application : IHC-P
Deng HS et al. Neurosci Lett 2019; 699:151-159 Proteomic profiling reveals Arl6ip-1 as a candidate target in cancer-induced bone pain rat model after * treatment.
Reactivity : Rat
Zbigniew Pietras et al. Nat Commun 2018; 9 (1):2558 Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria.
Application : IHC (Free Floating)
Reactivity : Mouse
Korovina I et al. FASEB J 2018;:fj201800430R Hematopoietic hypoxia-inducible factor 2α deficiency ameliorates pathological retinal neovascularization via modulation of endothelial cell apoptosis.
Reactivity : Mouse
E Bourseau-Guilmain et al. Nat Commun 2016; 7 (0):11371 Hypoxia regulates global membrane protein endocytosis through caveolin-1 in cancer cells.
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 379
$ 169