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Iba1 antibody [GT10312]

Anti-Iba1 antibody [GT10312] used in IHC (Frozen sections) (IHC-Fr). GTX632426
Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426
Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426
Anti-Iba1 antibody [GT10312] used in Flow cytometry (FACS). GTX632426
Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426
Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

Cat No. GTX632426

Reference 8
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT10312

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [GT10312] used in IHC (Frozen sections) (IHC-Fr). GTX632426

GTX632426 IHC-Fr Image

Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426

GTX632426 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426

GTX632426 ICC/IF Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 protein stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Iba1 antibody [GT10312] used in Flow cytometry (FACS). GTX632426

GTX632426 FACS Image

Iba1 antibody [GT10312] (GTX632426) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody [GT10312] (GTX632426) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Anti-Iba1 antibody [GT10312] used in Western Blot (WB). GTX632426

GTX632426 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [GT10312] used in IHC (Paraffin sections) (IHC-P). GTX632426

GTX632426 IHC-P Image

Iba1 antibody [GT10312] detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhong Z et al. Stem Cells Dev 2020; (Epub) Adipose-Derived Stem Cells Modulate BV2 Microglial M1/M2 Polarization by Producing Glial Cell-Derived Neurotrophic Factor.
Application : IHC-Fr
Reactivity : Mouse
Liu C et al. Neuroscience 2020; 432 : 84-93 Activation of the Notch Signaling Pathway and Cellular Localization of Notch Signaling Molecules in the Spinal Cord of SOD1-G93A ALS Model Mice.
Application : IHC-Fr
Reactivity : Mouse
Zhou X et al. Front Mol Neurosci 2019; 12 : 58 L-Cysteine-Derived H2S Promotes Microglia M2 Polarization via Activation of the AMPK Pathway in Hypoxia-Ischemic Neonatal Mice.
Van Weehaeghe D et al. J Nucl Med 2019; TSPO versus P2X7 as target for neuroinflammation - an in vitro and in vivo study.
Application : IHC-P
Reactivity : Human
Zhou X et al. Biomed Pharmacother 2019; 115 : 108818 Mesenchymal stem cell derived EVs mediate neuroprotection after spinal cord injury in rats via the microRNA-21-5p/FasL gene axis.
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Submit a Reference

REVIEW

Iba1 antibody [GT10312] Cat No. GTX632426

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Iba1 antibody [GT10312] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632426
Date : Tanusree Sen submitted on 26-Dec-2019
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : mouse microglia primary culture
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, 25°C, 0.5Hr
Primary Antibody : 1:1:100, 4°C, 12Hr
Submit a Review
Application Reference
Zhong Z et al. Stem Cells Dev 2020; (Epub) Adipose-Derived Stem Cells Modulate BV2 Microglial M1/M2 Polarization by Producing Glial Cell-Derived Neurotrophic Factor.
Application : IHC-Fr
Reactivity : Mouse
Liu C et al. Neuroscience 2020; 432:84-93 Activation of the Notch Signaling Pathway and Cellular Localization of Notch Signaling Molecules in the Spinal Cord of SOD1-G93A ALS Model Mice.
Application : IHC-Fr
Reactivity : Mouse
Zhou X et al. Front Mol Neurosci 2019; 12:58 L-Cysteine-Derived H2S Promotes Microglia M2 Polarization via Activation of the AMPK Pathway in Hypoxia-Ischemic Neonatal Mice.
Van Weehaeghe D et al. J Nucl Med 2019; TSPO versus P2X7 as target for neuroinflammation - an in vitro and in vivo study.
Application : IHC-P
Reactivity : Human
Zhou X et al. Biomed Pharmacother 2019; 115:108818 Mesenchymal stem cell derived EVs mediate neuroprotection after spinal cord injury in rats via the microRNA-21-5p/FasL gene axis.
Rosenzweig N et al. Nat Commun 2019; 10 (1):465 PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse model.
Application : IHC-P
Deng HS et al. Neurosci Lett 2019; 699:151-159 Proteomic profiling reveals Arl6ip-1 as a candidate target in cancer-induced bone pain rat model after * treatment.
Reactivity : Rat
Korovina I et al. FASEB J 2018;:fj201800430R Hematopoietic hypoxia-inducible factor 2α deficiency ameliorates pathological retinal neovascularization via modulation of endothelial cell apoptosis.
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329
$ 169