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Iba1 antibody [HL1880]

Recombinant antibody Comparison Orthogonal
Anti-Iba1 antibody [HL1880] used in Western Blot (WB). GTX637629
Anti-Iba1 antibody [HL1880] used in IHC (Paraffin sections) (IHC-P). GTX637629
Anti-Iba1 antibody [HL1880] used in IHC (Paraffin sections) (IHC-P). GTX637629
Anti-Iba1 antibody [HL1880] used in IHC (Frozen sections) (IHC-Fr). GTX637629
Anti-Iba1 antibody [HL1880] used in IHC (Frozen sections) (IHC-Fr). GTX637629
Anti-Iba1 antibody [HL1880] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637629
Anti-Iba1 antibody [HL1880] used in Western Blot (WB). GTX637629

Cat. No. GTX637629

Reference 2

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1880

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Observed MW

17 kDa.

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Iba1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [HL1880] used in Western Blot (WB). GTX637629

GTX637629 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL1880] (GTX637629) diluted at 1:1000 and competitor's antibody (CST17198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Iba1 antibody [HL1880] used in IHC (Paraffin sections) (IHC-P). GTX637629

GTX637629 IHC-P Image

Iba1 antibody [HL1880] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL1880] used in IHC (Paraffin sections) (IHC-P). GTX637629

GTX637629 IHC-P Image

Iba1 antibody [HL1880] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL1880] used in IHC (Frozen sections) (IHC-Fr). GTX637629

GTX637629 IHC-Fr Image

Iba1 antibody [HL1880] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL1880] used in IHC (Frozen sections) (IHC-Fr). GTX637629

GTX637629 IHC-Fr Image

Iba1 antibody [HL1880] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL1880] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637629

GTX637629 ICC/IF Image

Iba1 antibody [HL1880] detects Iba1 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [HL1880] (GTX637629) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL1880] used in Western Blot (WB). GTX637629

GTX637629 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL1880] (GTX637629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Hsu JC et al. Front Microbiol 2024; 15 : 1455462 Characterizing neuroinvasion and neuropathology of SARS-CoV-2 by using AC70 human ACE2 transgenic mice.
P Saenkham-Huntsinger et al. J Gen Virol 2024; 105 (10) : 2039 BALB/c mice challenged with SARS-CoV-2 B.1.351 β variant cause pathophysiological and neurological changes within the lungs and brains
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Package List Price ($)
$ 419
$ 169
Custom Conjugations