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Iba1 antibody [HL22-RT]

Recombinant antibody
Anti-Iba1 antibody [HL22-RT] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635400
Anti-Iba1 antibody [HL22-RT] used in Western Blot (WB). GTX635400
Anti-Iba1 antibody [HL22-RT] used in IHC (Paraffin sections) (IHC-P). GTX635400
Anti-Iba1 antibody [HL22-RT] used in IHC (Paraffin sections) (IHC-P). GTX635400
Anti-Iba1 antibody [HL22-RT] used in IHC (Frozen sections) (IHC-Fr). GTX635400

Cat. No. GTX635400

Host

 Rat

Clonality

 Monoclonal

Clone Name

 HL22-RT

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888528

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [HL22-RT] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635400

GTX635400 ICC/IF Image

Iba1 antibody [HL22-RT] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22-RT] used in Western Blot (WB). GTX635400

GTX635400 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Iba1 antibody [HL22-RT] used in IHC (Paraffin sections) (IHC-P). GTX635400

GTX635400 IHC-P Image

Iba1 antibody [HL22-RT] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22-RT] used in IHC (Paraffin sections) (IHC-P). GTX635400

GTX635400 IHC-P Image

Iba1 antibody [HL22-RT] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22-RT] used in IHC (Frozen sections) (IHC-Fr). GTX635400

GTX635400 IHC-Fr Image

Iba1 antibody [HL22-RT] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations