Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Iba1 antibody [HL22-RT] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Iba1 antibody [HL22-RT] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [HL22-RT] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [HL22-RT] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).