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Iba1 antibody [HL22]

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363
Anti-Iba1 antibody [HL22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

Cat No. GTX635363

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL22

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($369),
25 μl ($169)
PRODUCT

Summary

Iba1 antibody detects Iba1, a protein widely used by neuroscientists as a microglial marker and to gauge microglial activation. It is also expressed in macrophages. Discrimination between these two cell populations can be achieved via double staining with TMEM119 antibody (GTX134087), another useful microglia-specific marker, by identifying brain-residing microglia as Iba1+/TMEM119+ while CNS-infiltrating macrophages are Iba1+/TMEM119-.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Iba1 antibody [HL22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635363

GTX635363 ICC/IF Image

Iba1 antibody [HL22] detects Iba1 antibody [HL22] protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 antibody [HL22] stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

Iba1 antibody [HL22] Cat No. GTX635363

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Brain
Blocking : Donkey serum, 25°C, 1Hr
Primary Antibody : 1:300, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 369
$ 169