GeneTex
United States (US)

Iba1 antibody

GTX100042_41556_IHC_R_19011505_873.jpg
GTX100042_41556_WB_M_Brain_19011505_531.jpg
GTX100042_41556_20160901_WB_R_liver_19011505_264.jpg
GTX100042_41556_20161005_IHC-Fr_R_19011505_884.jpg
GTX100042_39476_20160720_IFA_19011506_534.jpg
GTX100042_39476_IHC_19011506_311.jpg
GTX100042_41556_WB_19011506_856.jpg
GTX100042_39476_20170303_FACS_19011506_665.jpg
GTX100042_41556_20160901_WB_M_liver_19011506_835.jpg
GTX100042_41556_IHC_M_19011506_335.jpg
GTX100042_IHC_19011506_589.jpg
GTX100042_39476_IHC_M_19011506_209.jpg

Cat No. GTX100042

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , mouse brain , mouse liver , rat liver , *Mouse brain

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AIF1 antibody, ionized calcium-binding adapter molecule 1 antibody, allograft inflammatory factor 1 antibody, interferon gamma responsive transcript antibody, protein G1 antibody

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100042_41556_IHC_R_19011505_873.jpg

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100042_41556_WB_M_Brain_19011505_531.jpg

GTX100042 WB Image

Iba1 antibody detects Iba1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
15 % SDS-PAGE
Iba1 antibody (GTX100042) dilution: 1:1000

GTX100042_41556_20160901_WB_R_liver_19011505_264.jpg

GTX100042 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.

GTX100042_41556_20161005_IHC-Fr_R_19011505_884.jpg

GTX100042 IHC-Fr Image

Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100042_39476_20160720_IFA_19011506_534.jpg

GTX100042 ICC/IF Image

Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:400.
Blue: Hoechst 33342 staining.

GTX100042_39476_IHC_19011506_311.jpg

GTX100042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GTX100042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Iba1 antibody detects Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.

GTX100042_41556_WB_19011506_856.jpg

GTX100042 WB Image

Iba1 antibody detects Iba1 protein by Western blot analysis.
A. 30 μg THP-1 whole cell lysate/extract
B. 30 μg HL-60 whole cell lysate/extract
15 % SDS-PAGE
Iba1 antibody (GTX100042) dilution: 1:5000

GTX100042_39476_20170303_FACS_19011506_665.jpg

GTX100042 FACS Image

Iba1 antibody (GTX100042) detects Iba1 by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX100042) dilution: 1:50.

GTX100042_41556_20160901_WB_M_liver_19011506_835.jpg

GTX100042 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.

GTX100042_41556_IHC_M_19011506_335.jpg

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100042_IHC_19011506_589.jpg

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma stroma.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100042_39476_IHC_M_19011506_209.jpg

GTX100042 IHC-P Image

Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain tissue.
Iba1 (GTX100042) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
The anti-Iba1 (GTX100042) specifically recognizes Iba1, a microglia marker, in murine brain tissues.

REFERENCE
REVIEW

Iba1 antibody Cat No. GTX100042

Rating ( Average 4.5 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jun-2018
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Brain
Fixation : 4% PFA, 4°C, 48Hr
Primary Antibody : 1:100, 4°C, overnightHr
Date : Anonymous submitted on 18-Mar-2013
Score :
Application Tested : IHC
Sample Species : Ms
Sample : Mouse brain
Blocking : NGS,BSA,Trition X-100, 25°C, 2Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1M citrate buffer(pH6.0), 95°C, 10minHr
Primary Antibody : 1:1000, 25°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169