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Iba1 antibody

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042
Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042
Anti-Iba1 antibody used in Flow cytometry (FACS). GTX100042
Anti-Iba1 antibody used in Western Blot (WB). GTX100042
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

Cat No. GTX100042

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IHC Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , mouse brain , mouse liver , rat liver , *Mouse brain

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

allograft inflammatory factor 1,AIF-1,IBA1,IRT-1,IRT1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Iba1 antibody detects Iba1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
15 % SDS-PAGE
Iba1 antibody (GTX100042) dilution: 1:1000

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.

Anti-Iba1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX100042

GTX100042 IHC-Fr Image

Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100042

GTX100042 ICC/IF Image

Iba1 antibody detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:400.
Blue: Hoechst 33342 staining.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1(GTX100042) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Iba1 antibody detects Iba1 in the cytosol of Kupffer cell in human hepatoma by immunohistochemical analysis.

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Iba1 antibody (GTX100042) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody used in Flow cytometry (FACS). GTX100042

GTX100042 FACS Image

Iba1 antibody (GTX100042) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX100042) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Anti-Iba1 antibody used in Western Blot (WB). GTX100042

GTX100042 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100042

GTX100042 IHC-P Image

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

Iba1 antibody Cat No. GTX100042

Rating ( Average 4.5 based on 2 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jun-2018
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : Brain
Fixation : 4% PFA, 4°C, 48Hr
Primary Antibody : 1:100, 4°C, 16Hr
Date : Anonymous submitted on 18-Mar-2013
Score :
Application Tested : IHC
Sample Species : Ms
Sample : Mouse brain
Blocking : NGS,BSA,Trition X-100, 25°C, 2Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1M citrate buffer(pH6.0), 95°C, 10minHr
Primary Antibody : 1:1000, 25°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169