GeneTex
United States (US)

Iba1 antibody

GTX101495_41885_20160901_WB_M_liver_19011506_703.jpg
GTX101495_41885_20170405_IHC-P_R_19011506_334.jpg
GTX101495_41885_20161005_IHC-Fr_R_19011506_954.jpg
GTX101495_41766_20150508_IP_19011506_480.jpg
GTX101495_41885_20190201_FACS_19022001_655.jpg
GTX101495_41885_20190111_IHC-P_M_1_19022001_198.jpg
GTX101495_41885_20190111_IHC-P_M_19022001_975.jpg
GTX101495_41885_20141229_WB_19011506_494.jpg
GTX101495_39506_CT_FACS_19011506_731.jpg
GTX101495_41885_20160901_WB_R_liver_19011506_611.jpg

Cat No. GTX101495

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

THP-1 , HL-60 , mouse liver, rat liver

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Iba1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Allograft Inflammatory Factor 1,Aif-1,Iba1,Irt-1,Irt1,Aif1

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101495_41885_20160901_WB_M_liver_19011506_703.jpg

GTX101495 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101495_41885_20170405_IHC-P_R_19011506_334.jpg

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at microglia in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 antibody (GTX101495) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101495_41885_20161005_IHC-Fr_R_19011506_954.jpg

GTX101495 IHC-Fr Image

Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (GTX101495) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101495_41766_20150508_IP_19011506_480.jpg

GTX101495 IP Image

Immunoprecipitation of Iba1 protein from K562 whole cell extracts using 5 μg of Iba1 antibody (GTX101495).
Western blot analysis was performed using Iba1 antibody (GTX101495).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101495_41885_20190201_FACS_19022001_655.jpg

GTX101495 FACS Image

Iba1 antibody (GTX101495) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody (GTX101495) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX101495_41885_20190111_IHC-P_M_1_19022001_198.jpg

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101495_41885_20190111_IHC-P_M_19022001_975.jpg

GTX101495 IHC-P Image

Iba1 antibody detects Iba1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody (GTX101495) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101495_41885_20141229_WB_19011506_494.jpg

GTX101495 WB Image

Iba1 antibody detects Iba1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101495_39506_CT_FACS_19011506_731.jpg

GTX101495 FACS Image

Flow cytometry on primary murine microglia cells, staining with Iba1 (GTX101495) antibody using 1.0 µg per 4×105 cells. GTX101495 (blue), Rabbit IgG (green) ,Unstained (red).


GTX101495_41885_20160901_WB_R_liver_19011506_611.jpg

GTX101495 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX101495) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Iba1 antibody Cat No. GTX101495

Rating ( Average 4.5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Brain tissue
Fixation : 4% PFA, 4°C, 48Hr
Primary Antibody : 1:100, 4°C, overnightHr
Date : Anonymous submitted on 20-Jan-2013
Score :
Application Tested : FACS
Sample Species : Ms
Sample : Primary murine microglia
Blocking : 2.5% horse serum in 1X PBS, 4°C, 1Hr
Fixation : 4% paraformaldehyde + 0.2% triton X-100
Primary Antibody : 1: 1.0 μg in 4×10*5 cells, 4°C, 1Hr
Figure Note : GTX101495 (blue), Rabbit IgG (green) ,Unstained (red)
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169