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Iba1 (isoform 1 and 3) antibody, C-term

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

Cat No. GTX89792

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-3μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89792-PEP).

Specificity/Sensitivity

This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Allograft Inflammatory Factor 1 , Aif-1 , Iba1 , Irt-1 , Irt1 , Aif1

Cellular Localization

Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Background

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792

GTX89792 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

GTX89792 IHC-P Image

IHC-P analysis of human spleen using GTX89792 Iba1 (isoform 1 and 3) antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

GTX89792 IHC-P Image

IHC-P analysis of human placenta using GTX89792 Iba1 (isoform 1 and 3) antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792

GTX89792 WB Image

WB analysis of various samples using GTX89792 Iba1 antibody, C-term.
Lane 1: Human Brain lysate
Lane 2: Mouse Brain lysate
Loading: 35μg per lane .

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

GTX89792 IHC-P Image

IHC-P analysis of mouse brain using GTX89792 Iba1 (isoform 1 and 3) antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 2μg/ml

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

GTX89792 IHC-P Image

IHC-P analysis of human lung using GTX89792 Iba1 (isoform 1 and 3) antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 5μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 369