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Iba1 (isoform 1 and 3) antibody, C-term

Orthogonal
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792
Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

Cat. No. GTX89792

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μg ($399)
See all Iba1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-3μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89792-PEP).

Product Note

This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89792

GTX89792 IHC-Fr Image

Iba1 (isoform 1 and 3) antibody, C-term detects Iba1 (isoform 1 and 3) protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 (isoform 1 and 3) stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Red: TMEM119, stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792

GTX89792 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89792

GTX89792 IHC-Fr Image

Iba1 (isoform 1 and 3) antibody, C-term detects Iba1 (isoform 1 and 3) protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 (isoform 1 and 3) stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Red: TMEM119, stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89792

GTX89792 IHC-Fr Image

Iba1 (isoform 1 and 3) antibody, C-term detects Iba1 (isoform 1 and 3) protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 (isoform 1 and 3) stained by Iba1 (isoform 1 and 3) antibody, C-term (GTX89792) diluted at 1:250.
Red: TMEM119, stained by TMEM119 antibody (GTX134087) diluted at 1:250.
Blue: Hoechst 33342 staining.

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in Western Blot (WB). GTX89792

GTX89792 WB Image

WB analysis of rat (Lane A) and mouse (Lane B) brain lysates (35μg protein in RIPA buffer) using GTX89792 Iba1 (isoform 1 and 3) antibody, C-term.
Dilution : 2μg/ml

Dilution : 2μg/ml

Anti-Iba1 (isoform 1 and 3) antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89792

GTX89792 IHC-P Image

IHC-P analysis of human spleen tissue using GTX89792 Iba1 (isoform 1 and 3) antibody, C-term.
Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 4μg/ml

Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6
Dilution : 4μg/ml

REFERENCE

Application Reference

F Gimeno-Ferrer et al. Sci Rep 2024; 14 (1) : 7224 Neuropathological changes in the TASTPM mouse model of Alzheimer’s disease and their relation to hyperexcitability and cortical spreading depolarization
Application : IHC-Fr
F Gimeno-Ferrer et al. Exp Neurol 2023; Cortical spreading depolarization is a potential target for rat brain
Application : IHC-Fr
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399