Iba1 (isoforms 1 + 3) antibody, C-term

Cat. No. GTX89048

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX89048 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-3μg/ml
IHC-P	2-4μg/ml
Note : IHC-P Human Cerebellum shows staining of astrocytes.

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89048-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YEEKAREKEKP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Gene ID: 199 AIF1
UniProt: P55008 AIF1

Research Area

DATA IMAGES

GTX89048 IHC-P Image

IHC-P analysis of human cerebellum using GTX89048 Iba1 (isoforms 1 + 3) antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml

GTX89048 WB Image

WB analysis of human lymph node lysate using GTX89048 Iba1 (isoforms 1 + 3) antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty