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Id1 antibody

Anti-Id1 antibody used in Western Blot (WB). GTX48657
Anti-Id1 antibody used in Western Blot (WB). GTX48657
Anti-Id1 antibody used in Western Blot (WB). GTX48657
Anti-Id1 antibody used in Western Blot (WB). GTX48657
Anti-Id1 antibody used in Western Blot (WB). GTX48657

Cat No. GTX48657

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Zebrafish
Package
100 μl ($319)

APPLICATION

Application Note

For WB: Use at a suggested starting dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user.

PROPERTIES

Form

Liquid

Buffer

50 mM Tris buffer, 140 mM NaCl, pH 7.4 containing 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Synthetic peptide derived from the C-terminal region of zebrafish ID1 (Genbank accession# NP_571320.1).

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

The Id proteins (inhibitors of DNA binding /differentiation) represent a family of dominant negative regulators of the basic helix-loop-helix transcription factors whose activities result in delayed cell differentiation and prolonged proliferation.

Research Area

DATA IMAGES

Anti-Id1 antibody used in Western Blot (WB). GTX48657

GTX48657 WB Image

Sec HPLC Profile

Anti-Id1 antibody used in Western Blot (WB). GTX48657

GTX48657 WB Image

Zf anti-Id1 WB shows differentiation between Id proteins.
Lane 1: loading control
Lane 2: Id1 in‐vitro TNT
Lane 3: Id3 in‐vitro TNT

Anti-Id1 antibody used in Western Blot (WB). GTX48657

GTX48657 WB Image

Lane 1,2: AmSO4 fractionated
Lane 3,4: HCIC Purified
Lane 5, 6: Protein A Purified

Anti-Id1 antibody used in Western Blot (WB). GTX48657

GTX48657 WB Image

Zf anti‐Id1 WB shows specificity amongst all other Zf embryo proteins
Lane 1: Id1 in‐vitro TNT
Lane 2: Standards
Lane 3: De‐yolked 24 hpf D. rerio embryo extract (band shift vs. lane 1 due to lack of glycosylation of TNT sample)

Anti-Id1 antibody used in Western Blot (WB). GTX48657

GTX48657 WB Image

Zf anti‐Id1 is specific.
(Left) CBB‐stained SDS PAGE.
(Right) Duplicate western blot
Lane 1: Standards
Lane 2: 1ml Id1 in‐vitro TNT

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf