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Idnk protein, His tag (active)

Idnk protein, His tag (active). GTX66895-pro

Cat No. GTX66895-pro

Application

Functional Assay

Species

E. coli
Package
20 μg ($219)

APPLICATION

Application Note

Specific activity is > 80 units/mg, in which one unit will convert 1.0 μmole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 0.15 M NaCl, 10% glycerol, 1 mM DTT.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

Full length protein, N-terminal His-Tag; Length: 210 a.a. Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE

Expression System

E.coli

Purity

> 90% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

D-gluconate kinase, thermosensitive , ECK4261 , JW4225 , gntV

Database

Research Area

DATA IMAGES

Idnk protein, His tag (active). GTX66895-pro

GTX66895-pro Image

3 μg of GTX66895-pro Idnk protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
Glycerol.pdf