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Ikaros antibody [1A12-F2-D8]

Anti-Ikaros antibody [1A12-F2-D8] used in Western Blot (WB). GTX49214
Anti-Ikaros antibody [1A12-F2-D8] used in Immunoprecipitation (IP). GTX49214

Cat No. GTX49214

Host

Mouse

Clonality

Monoclonal

Clone Name

1A12-F2-D8

Isotype

IgG1

Application

WB, IP

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IKAROS family zinc finger 1 , CVID13 , Hs.54452 , IK1 , IKAROS , LYF1 , LyF-1 , PPP1R92 , PRO0758 , ZNFN1A1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-Ikaros antibody [1A12-F2-D8] used in Western Blot (WB). GTX49214

GTX49214 WB Image

WB analysis of Ramos and Jurkat lysates using Ikaros antibody at a dilution of 1:1,000.

Anti-Ikaros antibody [1A12-F2-D8] used in Immunoprecipitation (IP). GTX49214

GTX49214 IP Image

IP analysis of HeLa lysate using control IgG and Ikaros antibody. The precipitates were detected by the same Ikaros antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339