GeneTex
United States (US)

Ikaros antibody, Internal

GTX88621_1959_WB_18122102_454.jpg

Cat No. GTX88621

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88621-PEP).

Specificity/Sensitivity

This antibody is expected to recognise all reported human isoforms.

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-DLLRAASENSQDALR, from the internal region of the protein sequence according to NP_006051.1; NP_001207694.1; NP_001207695.1; NP_001207696.1; NP_001207697.1; NP_001207698.1; NP_001207699.1; NP_001207700.1 and more.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IKAROS Antibody , PRO0758 Antibody , ZNFN1A1 Antibody , HS.54452 Antibody , IKZF1 Antibody , HIK1 Antibody , IK1 Antibody , LYF1 Antibody

Cellular Localization

In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events. In resting

Background

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. All isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homodimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and contain the nuclear localization signal, resulting in members with and without DNA-binding properties. Only few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isofor
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Database

Research Area

DATA IMAGES
GTX88621_1959_WB_18122102_454.jpg

GTX88621 WB Image

WB analysis of Molt-4 cell lysate using GTX88621 Ikaros antibody, Internal.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer