GeneTex
  • Country / Location Selection

United States (US)

Ikaros antibody [JB50-38]

Recombinant antibody
Anti-Ikaros antibody [JB50-38] used in Western Blot (WB). GTX01051
Anti-Ikaros antibody [JB50-38] used in Flow cytometry (FACS). GTX01051
Anti-Ikaros antibody [JB50-38] used in IHC (Paraffin sections) (IHC-P). GTX01051
Anti-Ikaros antibody [JB50-38] used in IHC (Paraffin sections) (IHC-P). GTX01051
Anti-Ikaros antibody [JB50-38] used in IHC (Paraffin sections) (IHC-P). GTX01051

Cat No. GTX01051

Host

Rabbit

Clonality

Monoclonal

Clone Name

JB50-38

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

48-75 kDa. The observed M.W. is based on the following publication. PMID: 15015965

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IKAROS family zinc finger 1,CVID13,Hs.54452,IK1,IKAROS,IKZF1,LYF1,LyF1,PPP1R92,PRO0758,ZNFN1A1,Ikaros

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-Ikaros antibody [JB50-38] used in Western Blot (WB). GTX01051

GTX01051 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ikaros antibody [JB50-38] (GTX01051) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed.It is possibly due to alternative splicing.
Reference: PMID: 15015965

Anti-Ikaros antibody [JB50-38] used in Flow cytometry (FACS). GTX01051

GTX01051 FACS Image

FACS analysis of Daudi cells using GTX01051 Ikaros antibody [JB50-38].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

Anti-Ikaros antibody [JB50-38] used in IHC (Paraffin sections) (IHC-P). GTX01051

GTX01051 IHC-P Image

IHC-P analysis of mouse spleen tissue section using GTX01051 Ikaros antibody [JB50-38].

Anti-Ikaros antibody [JB50-38] used in IHC (Paraffin sections) (IHC-P). GTX01051

GTX01051 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX01051 Ikaros antibody [JB50-38].

Anti-Ikaros antibody [JB50-38] used in IHC (Paraffin sections) (IHC-P). GTX01051

GTX01051 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX01051 Ikaros antibody [JB50-38].

REFERENCE

There are currently no references for Ikaros antibody [JB50-38] (GTX01051). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Ikaros antibody [JB50-38] (GTX01051). Be the first to share your experience with this product.
Package List Price ($)
$ 369