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Ikaros antibody

Anti-Ikaros antibody used in Western Blot (WB). GTX129438
Anti-Ikaros antibody used in Immunoprecipitation (IP). GTX129438
Anti-Ikaros antibody used in ChIP assay (ChIP assay). GTX129438

Cat No. GTX129438

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ChIP assay

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Ikaros. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

IKAROS family zinc finger 1 , CVID13 , Hs.54452 , IK1 , IKAROS , LYF1 , LyF-1 , PPP1R92 , PRO0758 , ZNFN1A1

Cellular Localization

Nucleus

Background

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs).

Database

Research Area

DATA IMAGES

Anti-Ikaros antibody used in Western Blot (WB). GTX129438

GTX129438 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ikaros antibody (GTX129438) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ikaros antibody used in Immunoprecipitation (IP). GTX129438

GTX129438 IP Image

IKZF1 antibody immunoprecipitates IKZF1 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of IKZF1 protein by 4 μg IKZF1 antibody (GTX129438)
7.5 % SDS-PAGE
The immunoprecipitated IKZF1 protein was detected by IKZF1 antibody (GTX129438) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-Ikaros antibody used in ChIP assay (ChIP assay). GTX129438

GTX129438 ChIP assay Image

Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-IKZF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.
ChIP experiment and primer designs are based on J Biol Chem. 2007 Jan 26;282(4):2538-47.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169