IKZF1 antibody immunoprecipitates IKZF1 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of IKZF1 protein by 4 μg IKZF1 antibody (GTX129438)
7.5 % SDS-PAGE
The immunoprecipitated IKZF1 protein was detected by IKZF1 antibody (GTX129438) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Cross-linked ChIP was performed with Jurkat chromatin extract and 5 μg of either control rabbit IgG or anti-IKZF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ikaros antibody (GTX129438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.