GeneTex
United States (US)

Importin 13 antibody [C3], C-term

GTX107982_IP_19011507_723.jpg
GTX107982_39757_WB_R_19011507_635.jpg
GTX107982_IFA_19011507_787.jpg
GTX107982_39757_IHC_19011507_590.jpg
GTX107982_WB_19011507_571.jpg

Cat No. GTX107982

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

HeLa , Importin 13 tansfected 293T cell

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Importin 13,Imp13,Kap13,Lgl2,Ranbp13,Ipo13

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A.

Database

DATA IMAGES
GTX107982_IP_19011507_723.jpg

GTX107982 IP Image

IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody (GTX107982).
The precipitated IPO13 was detected by GTX107982 diluted at 1:1000.

GTX107982_39757_WB_R_19011507_635.jpg

GTX107982 WB Image

Importin 13 antibody [C3], C-term detects Importin 13 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
Importin 13 antibody [C3], C-term (GTX107982) dilution: 1:1000

GTX107982_IFA_19011507_787.jpg

GTX107982 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Importin 13(GTX107982) antibody at 1:200 dilution.

GTX107982_39757_IHC_19011507_590.jpg

GTX107982 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using Importin 13(GTX107982) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107982_WB_19011507_571.jpg

GTX107982 WB Image

Western Blot analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody.
A: Non-transfected lysate.
B: IPO13 transfected lysate.
7.5% SDS PAGE
GTX107982 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169