GeneTex
United States (US)

Importin 13 antibody [N1N2], N-term

GTX115274_40282_IFA_18121410_539.jpg
GTX115274_40282_IHC_18121410_241.jpg
GTX115274_40282_WB_B_18121410_636.jpg

Cat No. GTX115274

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

human IPO13 transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Importin 13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Importin 13,Imp13,Kap13,Lgl2,Ranbp13,Ipo13

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A.

Database

DATA IMAGES
GTX115274_40282_IFA_18121410_539.jpg

GTX115274 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Importin 13(GTX115274) antibody at 1:200 dilution.

GTX115274_40282_IHC_18121410_241.jpg

GTX115274 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using Importin 13(GTX115274) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115274_40282_WB_B_18121410_636.jpg

GTX115274 WB Image

Importin 13 antibody detects Importin 13 protein by western blot analysis. Non-transfected (-) and Importin 13-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Importin 13 antibody (GTX115274) diluted by 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169