*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , Jurkat , NT2D1
Knockdown/Knockout validation was supported by references (PMID:25999477)
Mouse, Rat, Bovine(>80% identity)
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 7. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
FLJ14581 antibody, Imp7 antibody, MGC138673 antibody, RANBP7 antibody, IPO7 antibody, importin-7 antibody, RAN-binding protein 7 antibody, importin 7 antibody
Cytoplasm , Nucleus
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq]
GTX106408 IHC-P Image
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX106408 ICC/IF Image
Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IPO7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.
Blue: Hoechst 33343 staining.
GTX106408 ICC/IF Image
Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Importin 7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
GTX106408 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX106408 diluted at 1:1000