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Importin 7 antibody [C3], C-term

KO/KD
Anti-Importin 7 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106408
Anti-Importin 7 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106408
Anti-Importin 7 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106408
Anti-Importin 7 antibody [C3], C-term used in Western Blot (WB). GTX106408

Cat. No. GTX106408

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Importin 7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

Positive Control

Jurkat

Product Note

Knockdown/Knockout validation was supported by references (PMID:25999477)

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

importin 7 , Imp7 , RANBP7

Cellular Localization

Cytoplasm , Nucleus

Background

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Importin 7 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106408

GTX106408 ICC/IF Image

Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Importin 7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Importin 7 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106408

GTX106408 ICC/IF Image

Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IPO7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-Importin 7 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106408

GTX106408 IHC-P Image

Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Importin 7 antibody [C3], C-term used in Western Blot (WB). GTX106408

GTX106408 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX106408 diluted at 1:1000

REFERENCE

Application Reference

Jeong SA et al. J Cell Sci 2015; 128 (12) : 2287-301 Akt-mediated phosphorylation increases the binding affinity of hTERT for importin α to promote nuclear translocation.
Application : WB
Reactivity : Human
Lieu KG et al. J Cell Biol 2014; 205 (3) : 301-12 The p53-induced factor Ei24 inhibits nuclear import through an importin β-binding-like domain.
Application : ICC/IF
Reactivity : Human
Joubert DA et al. J Virol 2014; 88 (3) : 1591-603 Bovine ephemeral fever rhabdovirus α1 protein has viroporin-like properties and binds importin β1 and importin 7.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169