GeneTex
United States (US)

Importin 7 antibody [C3], C-term

KO/KD
GTX106408_39694_20141219_IHC_19011506_380.jpg
GTX106408_39694_IFA_19011506_167.jpg
GTX106408_39694_20150410_IFA_19011506_530.jpg
GTX106408_WB_19011506_867.jpg

Cat No. GTX106408

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

Positive Control

A549 , HeLa , Jurkat , NT2D1

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:25999477)

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ14581 antibody, Imp7 antibody, MGC138673 antibody, RANBP7 antibody, IPO7 antibody, importin-7 antibody, RAN-binding protein 7 antibody, importin 7 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106408_39694_20141219_IHC_19011506_380.jpg

GTX106408 IHC-P Image

Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106408_39694_IFA_19011506_167.jpg

GTX106408 ICC/IF Image

Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IPO7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX106408_39694_20150410_IFA_19011506_530.jpg

GTX106408 ICC/IF Image

Importin 7 antibody [C3], C-term detects Importin 7 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Importin 7 protein stained by Importin 7 antibody [C3], C-term (GTX106408) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX106408_WB_19011506_867.jpg

GTX106408 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
GTX106408 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169