Importin beta 1 antibody [3E9]

Cat. No. GTX22811

Reference 5

Host	Mouse
Clonality	Monoclonal
Clone Name	3E9
Isotype	IgG2a
Application	WB, ICC/IF, FACS, IP, ChIP assay, IHC, Neutralizing/Inhibition
Reactivity	Human, Mouse, Rat, Bovine, Dog, Pig
Package	100 μg ($399)

See all Importin beta 1 products

View product citations for antibody GTX22811 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5,000
ICC/IF	1:1000
FACS	1 μg/test
IP	Assay dependent
ChIP assay	Assay dependent
IHC	Assay dependent
Neutralizing/Inhibition	Assay dependent

Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Product Note

This antibody does not detect bacterially expressed recombinant NTF97. Immunofluorescence staining of NTF97 in MDBK cells with GTX22811 results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Purified NTF97 from bovine erythrocytes.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384867

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

karyopherin subunit beta 1 , IMB1 , IPO1 , IPOB , Impnb , NTF97

Cellular Localization

Cytoplasm

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

Database

Gene ID: 3837 KPNB1
UniProt: Q14974 KPNB1

Research Area

DATA IMAGES

GTX22811 ICC/IF Image

ICC/IF analysis of PTK cells using GTX22811 Importin beta 1 antibody [3E9].

GTX22811 FACS Image

FACS analysis of NIH-3T3 cells using GTX22811 Importin beta 1 antibody [3E9] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

GTX22811 FACS Image

FACS analysis of Jurkat cells using GTX22811 Importin beta 1 antibody [3E9] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

GTX22811 FACS Image

FACS analysis of PC12 cells using GTX22811 Importin beta 1 antibody [3E9] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde