APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:50-1:100 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 190-216 amino acids from the N-terminal region of human KPNB1.
Purification
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
karyopherin subunit beta 1 , IMB1 , IPO1 , IPOB , Impnb , NTF97
Cellular Localization
Cytoplasm
Background
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
Database
Research Area
DATA IMAGES
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GTX81705 IHC-P Image
IHC-P analysis of human brain tissue using GTX81705 Importin beta 1 antibody, N-term.
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GTX81705 FACS Image
FACS analysis of CEM cells using GTX81705 Importin beta 1 antibody, N-term.
Top histogram : negative control
Bottom histogram : CEM cells
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GTX81705 WB Image
WB analysis of Y79 cell lysate (35ug/lane) using GTX81705 Importin beta 1 antibody, N-term.
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REFERENCE
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REVIEW
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