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Influenza A virus H1N1 HA (Hemagglutinin) antibody

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

Cat No. GTX127357

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Influenza A virus ( H1N1)
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

WSN virus infected DF1 , *influenza virus (A/WSN/33) infected MDCK

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus H1N1 HA (Hemagglutinin) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2728683

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Hemagglutinin , Influenza A Virus HA

Research Area

DATA IMAGES

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357

GTX127357 ICC/IF Image

Influenza A Virus H1N1 Hemagglutinin (HA) antibody detects HA (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: HA (H1N1) protein stained by Influenza A Virus H1N1 Hemagglutinin (HA) antibody (GTX127357) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 HA (Hemagglutinin) antibody (GTX127357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

Influenza A virus H1N1 HA (Hemagglutinin) antibody Cat No. GTX127357

Rating ( Average 5 based on 9 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Chen-I Hsu submitted on 10-Sep-2021
Score :
Application Tested : WB
Sample Species : H1N1
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-HA (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were transfected with Influenza A virus (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28842649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017.PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017.PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017.PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017.PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 30631056
Date : Anonymous submitted on 27-Jul-2018
Score :
Application Tested : ICC/IF
Sample : H1N1 influenza virus A/PR/8/1934 infected MDCK cell
Blocking : 2%BSA in PBS(+), 25°C, 1Hr
Fixation : 3% PFA/100mM HEPES pH7.2 with 2mM MgCl2, 3.6mM CaCl2
Permeabilization /Antigen retrieval : 0.5% TritonX-100 in PBS(+)
Primary Antibody : 1:500, 25°C, 1Hr
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : H1N1
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:5000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus A/WSN/33 infected MDCK cells (MOI=1 9hr).Lane 2: MDCK cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf