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Influenza A virus H1N1 HA (Hemagglutinin) antibody

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357
Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

Cat. No. GTX127357

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, Multiplexing

Reactivity

Influenza A virus ( H1N1)
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus H1N1 HA (Hemagglutinin) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2728683

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Hemagglutinin , Influenza A Virus HA

Research Area

DATA IMAGES

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by -% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 HA (Hemagglutinin) antibody (GTX127357) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127357

GTX127357 ICC/IF Image

Influenza A Virus H1N1 Hemagglutinin (HA) antibody detects HA (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: HA (H1N1) protein stained by Influenza A Virus H1N1 Hemagglutinin (HA) antibody (GTX127357) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus H1N1 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127357

GTX127357 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 HA (Hemagglutinin) antibody (GTX127357) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus H1N1 HA (Hemagglutinin) antibody Cat. No. GTX127357

Rating ( Average 5 based on 9 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Chen-I Hsu submitted on 10-Sep-2021
Score :
Application Tested : WB
Sample Species : H1N1
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-HA (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were transfected with Influenza A virus (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28842649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28953980
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2017. PMID: 28953980
Date : Anonymous submitted on 27-Jul-2018
Score :
Application Tested : ICC/IF
Sample : H1N1 influenza virus A/PR/8/1934 infected MDCK cell
Blocking : 2%BSA in PBS(+), 25°C, 1Hr
Fixation : 3% PFA/100mM HEPES pH7.2 with 2mM MgCl2, 3.6mM CaCl2
Permeabilization /Antigen retrieval : 0.5% TritonX-100 in PBS(+)
Primary Antibody : 1:500, 25°C, 1Hr
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : H1N1
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:5000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus A/WSN/33 infected MDCK cells (MOI=1 9hr).Lane 2: MDCK cells.
Date : Anonymous submitted on
Score :
Application Tested : WB
Sample Species : H1N1
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30631056
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production