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Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288]

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

Cat No. GTX629696

Host

Mouse

Clonality

Monoclonal

Clone Name

GT288

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Influenza A virus ( H1N1)
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Influenza A (WSN) infected DF1 cell for 10 hr

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the Internal region of Influenza A virus H1N1 NA (Neuraminidase) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2728684

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Neuraminidase , Influenza A Virus NA

Research Area

DATA IMAGES

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

GTX629696 WB Image

Influenza A Virus H1N1 Neuraminidase (NA) antibody [GT288] detects Influenza A Virus H1N1 Neuraminidase (NA) protein by western blot analysis.
A. 30 μg DF1 whole cell lysate/extract (untreated)
B. 30 μg whole cell lysate/extract of Influenza A (WSN) infected DF1 cell for 10 hr
10% SDS-PAGE
Influenza A Virus H1N1 Neuraminidase (NA) antibody [GT288] (GTX629696) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] Cat No. GTX629696

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Viruses in 2019.PMID: 31500389
Date : Anonymous submitted on 16-Jun-2016
Score :
Application Tested : ICC/IF
Sample : influenza virus A/PR/8/1934 infected MDCK cells
Blocking : 2% BSA in PBS (plus Calcium, Magnesium), 25°C, 1Hr
Fixation : 3%PFA, 2mM MgCl2, 3.6mMCaCl2 in 100mM HEPES pH7.2
Permeabilization /Antigen retrieval : 0.5% TritonX-100 in PBS(+)
Primary Antibody : 1:500, 25°C, 1Hr
Application Reference
SDS
PBS.pdf