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Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288]

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696
Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

Cat. No. GTX629696

Host

Mouse

Clonality

Monoclonal

Clone Name

GT288

Isotype

IgG2a

Application

WB, ICC/IF

Reactivity

Influenza A virus ( H1N1)
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Influenza A (WSN) infected DF1 cell for 10 hr

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the Internal region of Influenza A virus H1N1 NA (Neuraminidase) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2728684

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Neuraminidase , Influenza A Virus NA

Research Area

DATA IMAGES

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

GTX629696 WB Image

Influenza A viral lysates were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] (GTX629696) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] used in Western Blot (WB). GTX629696

GTX629696 WB Image

Influenza A Virus H1N1 Neuraminidase (NA) antibody [GT288] detects Influenza A Virus H1N1 Neuraminidase (NA) protein by western blot analysis.
A. 30 μg DF1 whole cell lysate/extract (untreated)
B. 30 μg whole cell lysate/extract of Influenza A (WSN) infected DF1 cell for 10 hr
10% SDS-PAGE
Influenza A Virus H1N1 Neuraminidase (NA) antibody [GT288] (GTX629696) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] Cat. No. GTX629696

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Viruses in 2019.PMID: 31500389
Date : Anonymous submitted on 16-Jun-2016
Score :
Application Tested : ICC/IF
Sample : influenza virus A/PR/8/1934 infected MDCK cells
Blocking : 2% BSA in PBS (plus Calcium, Magnesium), 25°C, 1Hr
Fixation : 3%PFA, 2mM MgCl2, 3.6mMCaCl2 in 100mM HEPES pH7.2
Permeabilization /Antigen retrieval : 0.5% TritonX-100 in PBS(+)
Primary Antibody : 1:500, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations