GeneTex
United States (US)

Influenza A virus H1N1 NA (Neuraminidase) antibody

GTX125974_41164_WB_18121410_701.jpg
GTX125974_42732_20170119_WB_H1N1_18121410_642.jpg

Cat No. GTX125974

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Influenza A virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

DF-1 , WSN virus infected DF-1 , influenza A virus H1N1-infected H1299
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus Neuraminidase (NA) (A/WSN/1933(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Influenza A subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. There are 16 different HA antigens (H1-H16) and nine different NA antigens (N1-N9) for Influenza A. The extent of infection into host organisms is determined by HA, which interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues.

Research Area

DATA IMAGES
GTX125974_41164_WB_18121410_701.jpg

GTX125974 WB Image

Influenza A Virus H1N1 Neuraminidase (NA) antibody detects Influenza A Virus H1N1 Neuraminidase protein by western blot analysis.
A. 5 µg DF-1 whole cell lysate/extract
B. 5 µg whole cell lysate/extract WSN virus infected DF-1 cells
10% SDS-PAGE
Influenza A Virus H1N1 Neuraminidase (NA) antibody (GTX125974) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125974_42732_20170119_WB_H1N1_18121410_642.jpg

GTX125974 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody (GTX125974) diluted at 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169