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Influenza A virus H3N2 HA (Hemagglutinin) antibody

Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in IHC (Paraffin sections) (IHC-P). GTX127363
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363

Cat No. GTX127363

Reference 3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Influenza A virus
Package
100 μl ($319),
25 μl ($169)
See all Influenza A virus HA3 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:50-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

H3N2 HA recombinant protein
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus Hemagglutinin (HA) (A/TW/3446/02(H3N2)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in IHC (Paraffin sections) (IHC-P). GTX127363

GTX127363 IHC-P Image

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects Influenza A Virus H3N2 Hemagglutinin (HA) protein on mouse nasal tissue(infect with Influenza Virus A ) by immunohistochemical analysis.
Sample: Paraffin-embedded mouse nasal tissue(infect with Influenza Virus A ).
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:50.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363

GTX127363 WB Image

Recombinant H3N2 HA protein were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363

GTX127363 WB Image

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects HA (H3N2) protein by western blot analysis.
A. MDCK cell lysates infected with 4 HAU/ml of A/duck/Alberta/35/1976-like, H1N1
B. MDCK cell lysates infected with 4 HAU/ml of A/duck/Ukraine/1963-like, H3N8
C. MDCK cell lysates infected with 4 HAU/ml of A/mallard/Miyagi/53/1976-like, H5N3
D. MDCK cell lysates infected with 4 HAU/ml of A/duck/Taiwan/33/1993-like, H7N7
E. MDCK cell lysates infected with 4 HAU/ml of A/turkey/Wisconsin/1/1966-like, H9N2
F. MDCK cell lysates infected with 4 HAU/ml of A/PR/8/34, H1N1
G. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/126/2009, pdmH1N1
H. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/3446/2002, H3N2
12% SDS-PAGE
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Limburg H et al. J Virol 2019; TMPRSS2 is the major activating protease of influenza A virus in primary human airway cells and influenza B virus in human type II pneumocytes.
Diot C et al. Sci Rep 2016; 6 : 33763 Influenza A Virus Polymerase Recruits the RNA Helicase DDX19 to Promote the Nuclear Export of Viral mRNAs.
Application : WB
Xia C et al. J Virol 2015; Hemagglutinin of influenza A virus antagonizes type I IFN responses by inducing degradation of type I IFN receptor 1.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169