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Influenza A virus H3N2 HA (Hemagglutinin) antibody

Orthogonal
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in IHC (Paraffin sections) (IHC-P). GTX127363
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363

Cat No. GTX127363

Reference 6
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Influenza A virus
Package
100 μl ($379),
25 μl ($169)
See all Influenza A virus HA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:50-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

H3N2 HA recombinant protein

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus H3N2 HA (Hemagglutinin) (A/TW/3446/02(H3N2). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885648

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Hemagglutinin , Influenza A Virus HA

Research Area

DATA IMAGES

Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in IHC (Paraffin sections) (IHC-P). GTX127363

GTX127363 IHC-P Image

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects Influenza A Virus H3N2 Hemagglutinin (HA) protein on mouse nasal tissue(infect with Influenza Virus A ) by immunohistochemical analysis.
Sample: Paraffin-embedded mouse nasal tissue(infect with Influenza Virus A ).
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:50.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363

GTX127363 WB Image

Recombinant H3N2 HA protein were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H3N2 HA (Hemagglutinin) antibody (GTX127363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363

GTX127363 WB Image

Influenza A Virus H3N2 Hemagglutinin (HA) antibody detects HA (H3N2) protein by western blot analysis.
A. MDCK cell lysates infected with 4 HAU/ml of A/duck/Alberta/35/1976-like, H1N1
B. MDCK cell lysates infected with 4 HAU/ml of A/duck/Ukraine/1963-like, H3N8
C. MDCK cell lysates infected with 4 HAU/ml of A/mallard/Miyagi/53/1976-like, H5N3
D. MDCK cell lysates infected with 4 HAU/ml of A/duck/Taiwan/33/1993-like, H7N7
E. MDCK cell lysates infected with 4 HAU/ml of A/turkey/Wisconsin/1/1966-like, H9N2
F. MDCK cell lysates infected with 4 HAU/ml of A/PR/8/34, H1N1
G. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/126/2009, pdmH1N1
H. MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/3446/2002, H3N2
12% SDS-PAGE
Influenza A Virus H3N2 Hemagglutinin (HA) antibody (GTX127363) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Dorothea Bestle et al. J Virol 2021; 95 (20) : e0090621 Hemagglutinins of Avian Influenza Viruses Are Proteolytically Activated by TMPRSS2 in Human and Murine Airway Cells.
Sch?n J et al. PLoS Pathog 2021; 17 (4) : e1009490 Neuraminidase-associated plasminogen recruitment enables systemic spread of natural avian Influenza viruses H3N1.
Application : WB
Harbig A et al. J Biol Chem 2020; 295 (33) : 11388-11407 Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways.
Limburg H et al. J Virol 2019; TMPRSS2 is the major activating protease of influenza A virus in primary human airway cells and influenza B virus in human type II pneumocytes.
Diot C et al. Sci Rep 2016; 6 : 33763 Influenza A Virus Polymerase Recruits the RNA Helicase DDX19 to Promote the Nuclear Export of Viral mRNAs.
Application : WB
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Submit a Reference

REVIEW

Influenza A virus H3N2 HA (Hemagglutinin) antibody Cat No. GTX127363

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27653209
Anti-Influenza A virus H3N2 HA (Hemagglutinin) antibody used in Western Blot (WB). GTX127363
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27653209
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169