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Influenza A virus M1 (matrix protein) antibody

Orthogonal
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928

Cat No. GTX125928

Reference 35
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Influenza A virus
Package
100 μl ($359),
25 μl ($169)
See all Influenza A virus M1 (matrix protein) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates, *influenza virus (A/WSN/33) infected MDCK

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus M1 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171466

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Membrane 1 protein , Influenza A Virus M1

Background

Influenza A virus matrix 1, M1, is a critical protein required for assembly and budding. Hemagglutinin associates with M1 via its cytoplasmic tail and transmembrane domain. The M2 and nucleoprotein proteins are critical in the replication cycle of influenza viruses. The M2 channel protein is an essential component of the viral envelope because of its ability to form a highly selective, pH-regulated, proton-conducting channel. The M2 channel allows protons to enter the interior of the virus and acidification weakens the interaction of the M1 protein with the ribonuclear core.

Research Area

DATA IMAGES

Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928

GTX125928 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
15% SDS PAGE
GTX125928 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928

GTX125928 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus M1 (matrix protein) antibody (GTX125928) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hage A et al. Cell Rep 2022; 38 (10) : 110434 The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity.
Application : WB
Kung YA et al. mBio 2022; : e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Zhang B et al. Nat Biomed Eng 2021; 5 (11) : 1288-1305 Site-specific PEGylation of interleukin-2 enhances immunosuppression via the sustained activation of regulatory T cells.
Application : IHC-Fr
Yang F et al. J Virol 2021; 95 (9) Discovery of a Novel Specific Inhibitor Targeting Influenza A Virus Nucleoprotein with Pleiotropic Inhibitory Effects on Various Steps of the Viral Life Cycle.
Application : ICC/IF
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
Application : WB, ICC/IF
Show More +
Submit a Reference

REVIEW

Influenza A virus M1 (matrix protein) antibody Cat No. GTX125928

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Viruses in 2017. PMID: 29156632
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019. PMID: 31067454
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28842649
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX125928
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:30000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Submit a Review
Application Reference
Hage A et al. Cell Rep 2022; 38 (10):110434 The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity.
Application : WB
Kung YA et al. mBio 2022;:e0271721 Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.
Application : ICC/IF
Zhang B et al. Nat Biomed Eng 2021; 5 (11):1288-1305 Site-specific PEGylation of interleukin-2 enhances immunosuppression via the sustained activation of regulatory T cells.
Application : IHC-Fr
Yang F et al. J Virol 2021; 95 (9) Discovery of a Novel Specific Inhibitor Targeting Influenza A Virus Nucleoprotein with Pleiotropic Inhibitory Effects on Various Steps of the Viral Life Cycle.
Application : ICC/IF
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
Application : WB, ICC/IF
Sarto J.D. et al. bioRxiv 2021; (Epub) Phosphorylation of JIP4 at S730 presents anti-viral properties against influenza A virus infection.
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
Application : WB
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Powell H et al. PLoS Pathog 2020; 16 (6):e1008411 Neuraminidase antigenic drift of H3N2 clade 3c.2a viruses alters virus replication, enzymatic activity and inhibitory antibody binding.
Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.
Application : WB
Danielle E Anderson et al. bioRxiv 2020; (Epub) Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy.
Application : WB
David L Lin et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Guo M et al. Int J Mol Sci 2019; 20 (24) Antiviral Activity of Benzoic Acid Derivative NC-5 Against Influenza A Virus and Its Neuraminidase Inhibition.
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Zhang X et al. Viruses 2019; 11 (9) Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo.
Application : WB
Ren X et al. Front Microbiol 2019; 10:1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB, ICC/IF
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Zhao L et al. Mol Ther Nucleic Acids 2019; 14:509-519 The Downregulation of MicroRNA hsa-miR-340-5p in IAV-Infected A549 Cells Suppresses Viral Replication by Targeting RIG-I and OAS2.
Application : WB
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Hong JY et al. Antiviral Res 2019; 164:12-22 Highly immunogenic influenza virus-like particles containing B-cell-activating factor (BAFF) for multi-subtype vaccine development.
Application : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.
Application : WB, ICC/IF
Mizuta S et al. ChemMedChem 2018; (Epub) Synthesis of Trifluoromethyl-α,β-unsaturated Lactones and Pyrazolinones and Discovery of Influenza Virus Polymerase Inhibitors.
Application : WB
Pin Yu et al. Viruses 2017; 9 (11) Neurotropism In Vitro and Mouse Models of Severe and Mild Infection with Clinical Strains of Enterovirus 71.
Application : WB
Liu H et al. J Virol 2017; 92 (1):pii: e00858-17 Mutations in the Influenza A virus * protein enhance virus budding to complement lethal mutations in the M2 cytoplasmic tail.
Application : WB
Hu Y et al. Int J Mol Sci 2017; 18 (9) Chemical Genomics Approach Leads to the Identification of Hesperadin, an Aurora B Kinase Inhibitor, as a Broad-Spectrum Influenza Antiviral.
Application : WB
Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.
Application : WB
Hu Y et al. Antiviral Res 2017; 145:103-113 Discovery of dapivirine, a nonnucleoside HIV-1 reverse transcriptase inhibitor, as a broad-spectrum antiviral against both influenza A and B viruses.
Application : WB, ICC/IF
Sakai T et al. Sci Rep 2017; 7:45043 Influenza A virus hemagglutinin and neuraminidase act as novel motile machinery.
Jin J et al. Biomed Pharmacother 2017; 97:385-394 Oroxylin A suppresses influenza A virus replication correlating with neuraminidase inhibition and induction of IFNs.
Application : WB
Kerviel A et al. PLoS One 2016; 11 (11):e0165421 Involvement of an Arginine Triplet in * Matrix Protein Interaction with Membranes and in * Recruitment into Virus-Like Particles of the Influenza A(H1N1)pdm09 Virus.
Application : WB, ICC/IF
Zhu L et al. Virology 2016; 496:131-137 PLC-γ1 is involved in the inflammatory response induced by influenza A virus H1N1 infection.
Yu J et al. Antiviral Res 2016; 127:68-78 Antiviral activity of SA-2 against influenza A virus in-vitro/vivo and its inhibition of RNA polymerase.
Application : WB
Reactivity : Influenza A virus
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 359
$ 169