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Influenza A virus M2 (matrix protein) antibody

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951

Cat. No. GTX125951

Reference 29
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus M2 (matrix protein) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

WSN-infected DF-1 cell

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein corresponding to Influenza A virus M2 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170983

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Membrane 2 protein , Influenza A Virus M2

Research Area

DATA IMAGES

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (GTX125951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
15% SDS PAGE
GTX125951 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951

GTX125951 ICC/IF Image

Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: M2 protein stained by Influenza A Virus M2 antibody (GTX125951) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

REFERENCE

Application Reference

Han YJ et al. J Biomed Sci 2023; 30 (1) : 10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Wu W et al. Front Cell Infect Microbiol 2022; 12 : 904775 MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34) : e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Guangwen Wang et al. PLoS Pathog 2022; 18 (4) : e1010446 PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
Xin Mao et al. Evid Based Complement Alternat Med 2021; 2021 : 5547424 Essential Oil-Rich Chinese Formula Luofushan-Baicao Oil Inhibits the Infection of Influenza A Virus through the Regulation of NF-κB P65 and IRF3 Activation.
Application : WB
Show More +
Submit a Reference

REVIEW

Influenza A virus M2 (matrix protein) antibody Cat. No. GTX125951

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 25°C, 1Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2018.PMID: 29723291
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 27795429
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 27795429
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2017. PMID: 29312159
Submit a Review
Application Reference
Han YJ et al. J Biomed Sci 2023; 30 (1):10 Targeting influenza A virus by splicing inhibitor herboxidiene reveals the importance of subtype-specific signatures around splice sites.
Wu W et al. Front Cell Infect Microbiol 2022; 12:904775 MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
Guangwen Wang et al. PLoS Pathog 2022; 18 (4):e1010446 PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.
Xin Mao et al. Evid Based Complement Alternat Med 2021; 2021:5547424 Essential Oil-Rich Chinese Formula Luofushan-Baicao Oil Inhibits the Infection of Influenza A Virus through the Regulation of NF-κB P65 and IRF3 Activation.
Application : WB
Ulferts R et al. Cell Rep 2021; 37 (4):109899 Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation.
Hara H et al. Sci Rep 2021; 11 (1):10109 Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells.
Application : WB
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
Application : WB
Mruk K et al. Development 2020; 147 (12) Targeted cell ablation in zebrafish using optogenetic transcriptional control.
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Zhang Q et al. Microbes Infect 2020; (Epub) SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.
Application : WB
Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.
Application : WB
Omi J et al. Nat Commun 2020; 11 (1):162 The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection.
Application : WB
Wang R et al. Autophagy 2019; 15 (7):1163-1181 Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production.
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Ren X et al. Front Microbiol 2019; 10:1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB
Karen Mruk et al. bioRxiv 2019; (Epub) Targeted cell ablation in zebrafish using optogenetic transcriptional control.
Application : ICC/IF
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Wohlgemuth N et al. J Virol 2018; (Epub) Influenza A virus M2 protein apical targeting is required for efficient virus replication.
Chida J et al. PLoS Pathog 2018; 14 (5):e1007049 Prion protein protects mice from lethal infection with influenza A viruses.
Application : WB
Holzberg M et al. Front Microbiol 2017; 8:2426 Vemurafenib Limits Influenza A Virus Propagation by Targeting Multiple Signaling Pathways.
Su WC et al. J Virol 2017; (Epub) Ubiquitination of the Cytoplasmic Domain of Influenza A Virus M2 Protein is Crucial for Production of Infectious Virus Particles.
Qian W et al. Dev Comp Immunol 2017; 78:43113 duck * regulatory factor 1 acts as a positive regulator in duck innate antiviral response.
Application : WB
Choi J et al. Front. Pharmacol. 2017; (Epub) Eupatorium fortunei and Its Components Increase Antiviral Immune Responses against RNA Viruses.
Application : ICC/IF
Reactivity : Influenza A virus
Zhu P et al. J Virol 2016; (Epub) Host cellular protein TRAPPC6AΔ interacts with influenza A virus M2 protein and regulates viral propagation by modulating M2 trafficking.
Application : WB
Perwitasari O et al. J Virol 2014; 88 (17):10228-43 Verdinexor, a novel selective inhibitor of nuclear export, reduces influenza a virus replication in vitro and in vivo.
Application : ICC/IF
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169