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Influenza A virus M2 (matrix protein) antibody

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

Cat No. GTX125951

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Influenza A virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates , H1N1-infected H1299
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to Internal region of Influenza A virus M2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (GTX125951) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus M2 (matrix protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125951

GTX125951 ICC/IF Image

Influenza A Virus M2 antibody detects M2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: M2 protein stained by Influenza A Virus M2 antibody (GTX125951) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

Anti-Influenza A virus M2 (matrix protein) antibody used in Western Blot (WB). GTX125951

GTX125951 WB Image

Sample (50 µg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
15% SDS PAGE
GTX125951 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169