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Influenza A virus NP (nucleoprotein) antibody

Anti-Influenza A virus NP (nucleoprotein) antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

Cat No. GTX125989

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA

Reactivity

Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX629633, Detection: GTX125989 or Capture: GTX125989, Detection: GTX629633

Not tested in other applications.

Positive Control

H1N1-infected H1299 , DF1 (Influenza A virus treatment for 10hr) , *influenza virus (A/WSN/33) infected MDCK

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein fragment of Influenza A virus NP (nucleoprotein) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168364

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Nucleoprotein , Influenza A Virus NP

Research Area

DATA IMAGES

Anti-Influenza A virus NP (nucleoprotein) antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus NP (nucleoprotein) antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus NP (nucleoprotein) antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NP (nucleoprotein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

Influenza A Virus NP antibody detects NP (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NP (H1N1) protein stained by Influenza A Virus NP antibody (GTX125989) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) DF1 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus NP (nucleoprotein) antibody Cat No. GTX125989

Rating ( Average 5 based on 12 users reviews)
Application
Western Blot(WB) ( Average 5 based on 9 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-May-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 5 μL
Blocking : 3% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28842649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Virol J in 2020.PMID: 32321537
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018.PMID: 29492200
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Virol J in 2020.PMID: 32321537
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:30000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf