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Influenza A virus NP (nucleoprotein) antibody

Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

Cat No. GTX125989

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Influenza A virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

H1N1-infected H1299 , DF1 (Influenza A virus treatment for 10hr) , *influenza virus (A/WSN/33) infected MDCK
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant protein fragment of Influenza A Virus NP (A/WSN/1933(H1N1))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NP (nucleoprotein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

Influenza A Virus NP antibody detects NP (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NP (H1N1) protein stained by Influenza A Virus NP antibody (GTX125989) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

Anti-Influenza A virus NP (nucleoprotein) antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) DF1 whole cell extracts (5 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Influenza A virus NP (nucleoprotein) antibody Cat No. GTX125989

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:30000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf