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Influenza A virus NS1 (nonstructural protein) antibody

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

Cat No. GTX125990

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Influenza A virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates , *Influenza virus-infected monkey vero cells

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant protein fragment of Influenza A virus NS1 (A/WSN/1933(H1N1))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Non-structural protein 1 , Influenza A Virus NS1

Research Area

DATA IMAGES

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Sample (10 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
12% SDS PAGE
GTX125990 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Influenza A virus NS1 (nonstructural protein) antibody detects Influenza A virus NS1 (nonstructural protein) protein by western blot analysis. Non-infected (-) and Influenza A virus (H1N1)-infected (+) H1299 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

GTX125990 ICC/IF Image

Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (GTX125990) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

REFERENCE

REVIEW

Influenza A virus NS1 (nonstructural protein) antibody Cat No. GTX125990

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected monkey vero cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf