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Influenza A virus NS1 (nonstructural protein) antibody

Orthogonal
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

Cat. No. GTX125990

Reference 39
9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus NS1 (nonstructural protein) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

WSN-infected DF-1 cell s , H1N1-infected H1299 cell

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus NS1 (nonstructural protein) (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170327

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Non-structural protein 1 , Influenza A Virus NS1

Research Area

DATA IMAGES

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Sample (10 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
12% SDS PAGE
GTX125990 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Influenza A virus NS1 (nonstructural protein) antibody detects Influenza A virus NS1 (nonstructural protein) protein by western blot analysis. Non-infected (-) and Influenza A virus (H1N1)-infected (+) H1299 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

GTX125990 ICC/IF Image

Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (GTX125990) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Orthogonal

Influenza A viral lysate and Influenza B viral lysate (0.1 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:440. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Seoane R et al. Front Cell Infect Microbiol 2022; 12 : 960138 eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production.
Lee MC et al. Front Cell Infect Microbiol 2022; 12 : 998584 Influenza A virus NS1 protein represses antiviral immune response by hijacking NF-κB to mediate transcription of type III IFN.
Ling YH et al. Front Microbiol 2022; 13 : 895779 Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein.
Jiang J et al. iScience 2022; 25 (7) : 104607 LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination.
Huapeng Feng et al. Viruses 2022; 14 (4) KAP1 Positively Modulates Influenza A Virus Replication by Interacting with PB2 and NS1 Proteins in Human Lung Epithelial Cells.
Application : WB
Show More +
Submit a Reference

REVIEW

Influenza A virus NS1 (nonstructural protein) antibody Cat. No. GTX125990

Rating
( Average 5 based on 9 users reviews)
Application
Western Blot(WB)
( Average 5 based on 9 users reviews)
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Viruses in 2019.PMID: 31500389
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2018.PMID: 29723291
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31604929
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31604929
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2018. PMID: 30258431
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Date : Anonymous submitted on 06-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected monkey vero cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Show More +
Submit a Review
Application Reference
Seoane R et al. Front Cell Infect Microbiol 2022; 12:960138 eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production.
Lee MC et al. Front Cell Infect Microbiol 2022; 12:998584 Influenza A virus NS1 protein represses antiviral immune response by hijacking NF-κB to mediate transcription of type III IFN.
Ling YH et al. Front Microbiol 2022; 13:895779 Nucleoporin 85 interacts with influenza A virus PB1 and PB2 to promote its replication by facilitating nuclear import of ribonucleoprotein.
Jiang J et al. iScience 2022; 25 (7):104607 LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination.
Huapeng Feng et al. Viruses 2022; 14 (4) KAP1 Positively Modulates Influenza A Virus Replication by Interacting with PB2 and NS1 Proteins in Human Lung Epithelial Cells.
Application : WB
Amol Patil et al. J Virol 2021; 95 (6) Phosphorylation of Influenza A Virus NS1 at Serine 205 Mediates Its Viral Polymerase-Enhancing Function.
Application : WB
Meng-Ting Huang et al. Viruses 2021; 13 (5) Dual R108K and G189D Mutations in the NS1 Protein of A/H1N1 Influenza Virus Counteract Host Innate Immune Responses.
Application : WB
Vanessa Gerlt et al. Int J Mol Sci 2021; 22 (20) Cellular Protein Phosphatase 2A Regulates Cell Survival Mechanisms in Influenza A Virus Infection.
Application : WB
Tseng YY et al. Viruses 2021; 13 (10) Interaction between NS1 and Cellular MAVS Contributes to NS1 Mitochondria Targeting.
Application : ICC/IF
Nacken W et al. mBio 2021; 12 (5):e0219621 The Effector Domain of the Influenza A Virus Nonstructural Protein NS1 Triggers Host Shutoff by Mediating Inhibition and Global Deregulation of Host Transcription When Associated with Specific Structures in the Nucleus.
Application : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Burke JM et al. Sci Adv 2021; 7 (23) RNase L limits host and viral protein synthesis via inhibition of mRNA export.
Application : ICC/IF
Sarto J.D. et al. bioRxiv 2021; (Epub) Phosphorylation of JIP4 at S730 presents anti-viral properties against influenza A virus infection.
Kwon EB et al. Molecules 2021; 26 (4) 8-O-(E-p-methoxycinnamoyl)harpagide Inhibits Influenza A Virus Infection by Suppressing Intracellular Calcium.
Application : WB
Sha TW et al. Virol J 2020; 17 (1):107 Influenza A virus NS1 optimises virus infectivity by enhancing genome packaging in a dsRNA-binding dependent manner.
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Du Y et al. Nat Commun 2020; 11 (1):2449 mRNA display with library of even-distribution reveals cellular interactors of influenza virus NS1.
Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.
Application : WB
Zhang T et al. Cell 2020; 180 (6):1115-1129 Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis.
Application : WB
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
Zhang X et al. Viruses 2019; 11 (9) Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo.
Application : WB
Ren X et al. Front Microbiol 2019; 10:1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Furusawa Y et al. MBio 2019; 10 (5) Influenza Virus Polymerase Mutation Stabilizes a Foreign Gene Inserted into the Virus Genome by Enhancing the Transcription/Replication Efficiency of the Modified Segment.
Application : WB
Moriyama M et al. Nat Commun 2019; 10 (1):4624 Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses.
Application : ICC/IF
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Sethy B et al. J Med Chem 2019; 62 (5):2390-2403 Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents.
Application : WB
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Russell AB et al. Elife 2018; 7 Extreme heterogeneity of influenza virus infection in single cells.
Chiba S et al. Front Microbiol 2018; 9:2192 The Cellular DExD/H-Box RNA Helicase UAP56 Co-localizes With the Influenza A Virus NS1 Protein.
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Chida J et al. PLoS Pathog 2018; 14 (5):e1007049 Prion protein protects mice from lethal infection with influenza A viruses.
Application : WB
Coates BM et al. J Immunol 2018; (Epub) Inflammatory Monocytes Drive Influenza A Virus-Mediated Lung Injury in Juvenile Mice.
Application : IHC-P
Hu Y et al. Int J Mol Sci 2017; 18 (9) Chemical Genomics Approach Leads to the Identification of Hesperadin, an Aurora B Kinase Inhibitor, as a Broad-Spectrum Influenza Antiviral.
Application : WB
Hu Y et al. Antiviral Res 2017; 145:103-113 Discovery of dapivirine, a nonnucleoside HIV-1 reverse transcriptase inhibitor, as a broad-spectrum antiviral against both influenza A and B viruses.
Application : WB
Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.
Application : WB
Reactivity : Influenza A virus
Tawaratsumida K et al. J Virol 2014; 88 (16):9038-48 Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor.
Application : WB
Wu NC et al. J Virol 2014; high-throughput identification of loss-of-function mutations for anti-* activity in influenza a virus ns segment.
Application : WB
Reactivity : Influenza A virus
Strengert M et al. Antioxid Redox Signal 2013; Mucosal Reactive Oxygen Species Are Required for Antiviral Response: Role of Duox in Influenza A Virus Infection.
Application : WB
Reactivity : Influenza A virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169