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Influenza A virus NS1 (nonstructural protein) antibody

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

Cat No. GTX125990

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Influenza A virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates , *Influenza virus-infected monkey vero cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant protein fragment of Influenza A virus NS1 (A/WSN/1933(H1N1))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Sample (10 µg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
12% SDS PAGE
GTX125990 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Western Blot (WB). GTX125990

GTX125990 WB Image

Influenza A virus NS1 (nonstructural protein) antibody detects Influenza A virus NS1 (nonstructural protein) protein by western blot analysis. Non-infected (-) and Influenza A virus (H1N1)-infected (+) H1299 whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NS1 (nonstructural protein) antibody (GTX125990) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus NS1 (nonstructural protein) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125990

GTX125990 ICC/IF Image

Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (GTX125990) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

REFERENCE
REVIEW

Influenza A virus NS1 (nonstructural protein) antibody Cat No. GTX125990

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected monkey vero cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169