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Influenza A virus NS2 / NEP antibody

Orthogonal
Anti-Influenza A virus NS2 / NEP antibody used in Western Blot (WB). GTX125953
Anti-Influenza A virus NS2 / NEP antibody used in Western Blot (WB). GTX125953

Cat No. GTX125953

Reference 10
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($379),
25 μl ($169)
See all Influenza A virus NS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus NS2 (nonstructural protein) (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175928

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Non-structural protein 2 , Influenza A Virus NS2

Background

Influenza A viruses are single stranded, negative sense RNA viruses. Two distinct non-structural proteins NS1 and NS2 are translated from one of the eight RNA segments by using different reading frames.The influenza A virus NS2 is located in the nucleus and the cytoplasm. NS2 is essential for nuclear export of influenza virus ribonucleoprotein (RNP) complexes.

Research Area

DATA IMAGES

Anti-Influenza A virus NS2 / NEP antibody used in Western Blot (WB). GTX125953

GTX125953 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus NS2 (nonstructural protein) antibody (GTX125953) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-Influenza A virus NS2 / NEP antibody used in Western Blot (WB). GTX125953

GTX125953 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
15% SDS PAGE
GTX125953 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhu Y et al. Sci Adv 2020; 6 (25) : eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Zhang X et al. Viruses 2019; 11 (9) Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo.
Application : WB
Ren X et al. Front Microbiol 2019; 10 : 1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Choi JG et al. Sci Rep 2019; 9 (1) : 12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Emmanuel Than-Trong et al. Development 2018; 145 (10) Neural stem cell quiescence and stemness are molecularly distinct outputs of the Notch3 signalling cascade in the vertebrate adult brain.
Application : WB
Show More +
Submit a Reference

REVIEW

Influenza A virus NS2 / NEP antibody Cat No. GTX125953

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus NS2 / NEP antibody used in Western Blot (WB). GTX125953
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Development in 2018. PMID: 29695612
Anti-Influenza A virus NS2 / NEP antibody used in Western Blot (WB). GTX125953
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2017. PMID: 29312159
Anti-Influenza A virus NS2 / NEP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125953
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25119991
Submit a Review
Application Reference
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Zhang X et al. Viruses 2019; 11 (9) Brevilin A, a Sesquiterpene Lactone, Inhibits the Replication of Influenza A Virus In Vitro and In Vivo.
Application : WB
Ren X et al. Front Microbiol 2019; 10:1625 Avian Influenza A Virus Polymerase Recruits Cellular RNA Helicase eIF4A3 to Promote Viral mRNA Splicing and Spliced mRNA Nuclear Export.
Application : WB
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Emmanuel Than-Trong et al. Development 2018; 145 (10) Neural stem cell quiescence and stemness are molecularly distinct outputs of the Notch3 signalling cascade in the vertebrate adult brain.
Application : WB
Holzberg M et al. Front Microbiol 2017; 8:2426 Vemurafenib Limits Influenza A Virus Propagation by Targeting Multiple Signaling Pathways.
Chutiwitoonchai N et al. Virology 2017; 507:32-39 Inhibition of CRM1-mediated nuclear export of influenza A nucleoprotein and nuclear export protein as a novel target for antiviral drug development.
Application : ICC/IF
Tawaratsumida K et al. J Virol 2014; 88 (16):9038-48 Quantitative proteomic analysis of the influenza A virus nonstructural proteins NS1 and NS2 during natural cell infection identifies PACT as an NS1 target protein and antiviral host factor.
Application : WB
Chutiwitoonchai N et al. PLoS One 2014; 9 (8):e105081 Comparative analysis of seven viral nuclear export signals (NESs) reveals the crucial role of nuclear export mediated by the third NES consensus sequence of nucleoprotein (NP) in influenza A virus replication.
Application : ICC/IF
Strengert M et al. Antioxid Redox Signal 2013; Mucosal Reactive Oxygen Species Are Required for Antiviral Response: Role of Duox in Influenza A Virus Infection.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169