GeneTex
United States (US)

Influenza A virus NS2 (nonstructural protein) antibody

GTX125953_40968_WB_WSN virus_18121410_389.jpg

Cat No. GTX125953

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Influenza A virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus NS2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Influenza A viruses are single stranded, negative sense RNA viruses. Two distinct non-structural proteins NS1 and NS2 are translated from one of the eight RNA segments by using different reading frames.The influenza A virus NS2 is located in the nucleus and the cytoplasm. NS2 is essential for nuclear export of influenza virus ribonucleoprotein (RNP) complexes.

Research Area

DATA IMAGES
GTX125953_40968_WB_WSN virus_18121410_389.jpg

GTX125953 WB Image

Sample (5 µg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
15% SDS PAGE
GTX125953 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169