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Influenza A virus Nucleoprotein antibody [C43]

Anti-Influenza A virus Nucleoprotein antibody [C43] used in Western Blot (WB). GTX00858
Anti-Influenza A virus Nucleoprotein antibody [C43] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00858
Anti-Influenza A virus Nucleoprotein antibody [C43] used in Western Blot (WB). GTX00858
Anti-Influenza A virus Nucleoprotein antibody [C43] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00858

Cat. No. GTX00858

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C43

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Influenza A virus
Package
100 μg ($399)
See all Influenza A virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1:1000
ICC/IF 1:200
IP 1:100
ELISA Assay dependent
Not tested in other applications.

Product Note

It reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34). No cross reactivity with influenza B viruses.
We do not recommend use of this product for Influenza B virus samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Human Influenza A Virus H3N2

Purification

Purified IgG
From culture supernant

Purity

90-95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody [C43] used in Western Blot (WB). GTX00858

GTX00858 WB Image

WB analysis of MDCK cells infected with Influenza A virus (H1N1, PuertoRico/8/34) using GTX00858 Influenza A virus NP (nucleoprotein) antibody [C43].
Dilution : 1:1000
SDS-PAGE : 10-20% gradient gel

Anti-Influenza A virus Nucleoprotein antibody [C43] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00858

GTX00858 ICC/IF Image

ICC/IF analysis of 293T cells were overexpressed with HA/NP protein of influenza A virus (H1N1, A/Suita/1/2009) using GTX00858 Influenza A virus NP (nucleoprotein) antibody [C43].
C43: Primaty antibody
anti-HA: a commercial HA monoclonal antibody

Anti-Influenza A virus Nucleoprotein antibody [C43] used in Western Blot (WB). GTX00858

GTX00858 WB Image

WB analysis of MDCK cells infected with various Influenza A virus strains and collected the samples in the indicated time points using GTX00858 Influenza A virus NP (nucleoprotein) antibody [C43].

Anti-Influenza A virus Nucleoprotein antibody [C43] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00858

GTX00858 ICC/IF Image

ICC/IF analysis of MDCK/A549 cells were infected with influenza A virus (H1N1, A/PuertoRico/8/34) using GTX00858 Influenza A virus NP (nucleoprotein) antibody [C43].
Fixation : 4% PFA in PBS
Permeabilization : 0.1% Triton X-100 in PBS

REFERENCE

Application Reference

Soni S et al. Virol J 2023; 20 (1) : 78 Independent role of caspases and Bik in augmenting influenza A virus replication in airway epithelial cells and mice.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399