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Influenza A virus Nucleoprotein antibody [GT1236]

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Western Blot (WB). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

Cat No. GTX629633

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1236

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

Influenza A virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX629633, Detection: GTX125989 or Capture: GTX125989, Detection: GTX629633

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

WSN virus infected DF1 cells (10 hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888128

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Nucleoprotein , Influenza A Virus NP

Research Area

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

GTX629633 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Western Blot (WB). GTX629633

GTX629633 WB Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by Western blot analysis.
A. 5 μg DF1 whole cell lysate/extract
B. 5 μg whole cell lysate/extract of WSN virus infected DF1 cells (10 hr)
7.5 % SDS-PAGE
Influenza A Virus NP antibody [GT1236] (GTX629633) dilution: 1:5000

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629633

GTX629633 ICC/IF Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by immunofluorescent analysis.
Sample: DF1 cells infected with Influenza A virus (WSN) .
Green: Influenza A Virus NP protein stained by Influenza A Virus NP antibody [GT1236] (GTX629633) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf