Influenza A virus Nucleoprotein antibody [GT1236]

Cat No. GTX629633

Reference 11

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1236
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA
Reactivity	Influenza A virus
Package	100 μl ($379), 25 μl ($169)

See all Influenza A virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IHC-Fr	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture: GTX629633, Detection: GTX125989　or Capture: GTX125989, Detection: GTX629633

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

WSN virus infected DF1 cells (10 hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888128

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Nucleoprotein , Influenza A Virus NP

Research Area

DATA IMAGES

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX629633 WB Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by Western blot analysis.
A. 5 μg DF1 whole cell lysate/extract
B. 5 μg whole cell lysate/extract of WSN virus infected DF1 cells (10 hr)
7.5 % SDS-PAGE
Influenza A Virus NP antibody [GT1236] (GTX629633) dilution: 1:5000

GTX629633 ICC/IF Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by immunofluorescent analysis.
Sample: DF1 cells infected with Influenza A virus (WSN) .
Green: Influenza A Virus NP protein stained by Influenza A Virus NP antibody [GT1236] (GTX629633) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX629633 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

Application Reference

Lee JH et al. Viruses 2021; 13 (9) Nonstructural Protein NS1 of Influenza Virus Disrupts Mitochondrial Dynamics and Enhances Mitophagy via ULK1 and BNIP3.

Application : WB

Zhang B et al. Nat Biomed Eng 2021; 5 (11) : 1288-1305 Site-specific PEGylation of interleukin-2 enhances immunosuppression via the sustained activation of regulatory T cells.

Application : IHC-Fr

Melano I et al. Viruses 2021; 13 (7) Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection.

Application : WB

Oh SJ et al. Cells 2021; 10 (3) SARS-CoV-2 Nucleocapsid Protein Targets RIG-I-Like Receptor Pathways to Inhibit the Induction of Interferon Response.

Application : WB

Lai Y et al. Front Pharmacol 2021; 12 : 657826 Antiviral Activity of Isoimperatorin Against Influenza A Virus in vitro and its Inhibition of Neuraminidase.

Application : WB

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REVIEW

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Application Reference

Lee JH et al. Viruses 2021; 13 (9) Nonstructural Protein NS1 of Influenza Virus Disrupts Mitochondrial Dynamics and Enhances Mitophagy via ULK1 and BNIP3.

Application : WB

Zhang B et al. Nat Biomed Eng 2021; 5 (11):1288-1305 Site-specific PEGylation of interleukin-2 enhances immunosuppression via the sustained activation of regulatory T cells.

Application : IHC-Fr

Melano I et al. Viruses 2021; 13 (7) Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection.

Application : WB

Oh SJ et al. Cells 2021; 10 (3) SARS-CoV-2 Nucleocapsid Protein Targets RIG-I-Like Receptor Pathways to Inhibit the Induction of Interferon Response.

Application : WB

Lai Y et al. Front Pharmacol 2021; 12:657826 Antiviral Activity of Isoimperatorin Against Influenza A Virus in vitro and its Inhibition of Neuraminidase.

Application : WB