*Optimal dilutions/concentrations should be determined by the researcher.
Application
Dilution
WB
1:500-1:3000
Not tested in other applications.
Positive Control
Influenza A virus (WSN) infected DF1
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.08 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Influenza A virus
Immunogen
Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888122
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Influenza A Virus Nucleoprotein , Influenza A Virus NP
Influenza A Virus NP antibody [GT778] detects Influenza A Virus NP protein by western blot analysis. A. 5 μg DF1 whole cell lysate/extract B. 5 μg of Influenza A virus (WSN) infected DF1 cells 7.5 % SDS-PAGE Influenza A Virus NP antibody [GT778] (GTX629544) dilution: 1:1000
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GTX629544 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [GT778] (GTX629544) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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