GeneTex
  • Country / Location Selection

United States (US)

Influenza A virus Nucleoprotein antibody [HL1078]

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199

Cat No. GTX636199

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1078

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Influenza A virus
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636318, Detection : GTX636199 or Capture : GTX636199, Detection : GTX636318.
Please notice that GTX636199 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. A Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Influenza A virus Nucleoprotein (A/Kansas/14/2017(H3N2)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909951

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199

GTX636199 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199

GTX636199 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636199).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX636199) diluted at 1:100.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199

GTX636199 WB Image

Influenza A viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 μg/mL).

Please notice that GTX636199 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).

Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636199. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199

GTX636199 ICC/IF Image

Influenza A virus Nucleoprotein antibody (GTX636199) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636199) diluted at 1:2000.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199

GTX636199 Lateral Flow Image

Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

REFERENCE

There are currently no references for Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199). Be the first to share your publications with this product.

REVIEW

Influenza A virus Nucleoprotein antibody [HL1078] Cat No. GTX636199

Rating ( Average 5 based on 1 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : Influenza A virus
Sample : influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)
Figure Note : Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Package List Price ($)
$ 369
$ 169
Custom Conjugations