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Influenza A virus Nucleoprotein antibody [HL1078]

Recombinant antibody Comparison Orthogonal
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199

Cat. No. GTX636199

Reference 1
1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1078

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture : GTX636199 / GTX637790, Detection : GTX637790 / GTX636199.
Please notice that the detection antibodies need to be conjugated to Gold to function when paired with capture antibodies. Please contact us for custom Gold-conjugated antibody.

Sandwich ELISA
Capture : GTX636318 / GTX636199, Detection : GTX636199 / GTX636318. Please notice that GTX636199 / GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318 / GTX636199. A Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.

Product Note

This antibody is specific for Influenza A virus Nucleoprotein protein (H1N1, H3N2, H5N8, and H10N3), and it does not cross-react with Influenza B virus Nucleoprotein protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Influenza A virus Nucleoprotein (A/Kansas/14/2017(H3N2)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909951

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Comparison

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199

GTX636199 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).

Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636199. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199

GTX636199 Lateral Flow Image

Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199

GTX636199 Lateral Flow Image

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.

Capture:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])
A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])
A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])
B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (40 ng) :
1. Lysis buffer
2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)
3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)
7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199

GTX636199 Lateral Flow Image

Detection of influenza A virus nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
Detection: Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])

Samples (1 ng) :
1. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
2. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
3. Lysis buffer

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199

GTX636199 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Western Blot (WB). GTX636199

GTX636199 WB Image

Influenza A viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199

GTX636199 ICC/IF Image

Influenza A virus Nucleoprotein antibody (GTX636199) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636199) diluted at 1:2000.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in ELISA (ELISA). GTX636199

GTX636199 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 μg/mL).

Please notice that GTX636199 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199

GTX636199 Lateral Flow Image

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.
Pair for S :
Capture : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688)
Detection : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689)
Pair for A:
Capture : Influenza A virus Nucleoprotein antibody [HL1952] (GTX637789)
Detection : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
Pair for B :
Capture : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Detection : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Samples (40 ng) :
1. Lysis buffer
2. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636199

GTX636199 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636199).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX636199) diluted at 1:100.

REFERENCE

Application Reference

N Miyakaw et al. Microchemical Journal 2023; Semi-quantitative graphene chemiresistor enzyme immunoassay for simple and sensitive antigen detection
Submit a Reference

REVIEW

Influenza A virus Nucleoprotein antibody [HL1078] Cat. No. GTX636199

Rating
( Average 5 based on 1 users reviews)
Application
Lateral Flow(Lateral Flow)
( Average 5 based on 1 users reviews)
Anti-Influenza A virus Nucleoprotein antibody [HL1078] used in Lateral Flow (Lateral Flow). GTX636199
Date : Anonymous submitted on 02-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : Influenza A virus
Sample : influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)
Figure Note : Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations