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Influenza A virus Nucleoprotein antibody

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989
Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

Cat. No. GTX125989

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA

Reactivity

Influenza A virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX629633, Detection: GTX125989 or Capture: GTX125989, Detection: GTX629633

Not tested in other applications.

Positive Control

DF1 (Influenza A virus treatment for 10hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168364

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Nucleoprotein , Influenza A Virus NP

Research Area

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) DF1 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Influenza A viral lysate (1 ug & 1ng μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in ELISA (ELISA). GTX125989

GTX125989 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

Influenza A virus Nucleoprotein antibody detects Influenza A virus Nucleoprotein protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Influenza A virus lysate (H1N1) infected 293T whole cell extract was separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125989

GTX125989 ICC/IF Image

Immunofluorescent analysis of Influenza virus infected cells using Influenza A virus Nucleoprotein antibody antibody (GTX125989).
Sample: Influenza A and B virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody antibody (GTX125989) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody used in Western Blot (WB). GTX125989

GTX125989 WB Image

Non-infected (–) and infected (+) H1299 whole cell extract (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody (GTX125989) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus Nucleoprotein antibody Cat. No. GTX125989

Rating ( Average 5 based on 22 users reviews)
Application
Western Blot(WB) ( Average 5 based on 19 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the 2022 in Cell Death Discov. PMID: 35246529
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-NP (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015.PMID: 26030097
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018.PMID: 29904383
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018.PMID: 29904383
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2018.PMID: 29904383
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 14-May-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 5 μL
Blocking : 3% BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28842649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017. PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017. PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017. PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017. PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Virol J in 2020. PMID: 32321537
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2018. PMID: 29492200
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017. PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017. PMID: 29312300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal Virol J in 2020. PMID: 32321537
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : influenza virus (A/WSN/33) infected MDCK cells
Amount used : 3 x 10^3 cells
Blocking : 5% nonfat drymilk in TBST, 25°C, overnightHr
Primary Antibody : 1:30000, 25°C, 4Hr
Lane Description : Lane 1: influenza virus (A/WSN/33) infected MDCK cells (MOI=1 9hr). Lane 2: MDCK cells.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169