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Influenza A virus PA protein antibody

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

Cat No. GTX125932

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Influenza A virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus PA (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932

GTX125932 ICC/IF Image

Influenza A Virus PA antibody detects PA protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PA protein stained by Influenza A Virus PA antibody (GTX125932) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Sample (5 µg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125932 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX125932) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169