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Influenza A virus PA protein antibody

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

Cat. No. GTX125932

Reference 26
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus PA protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

WSN virus infected DF1

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PA (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175740

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Polymerase acidic protein , Influenza A Virus PA

Research Area

DATA IMAGES

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932

GTX125932 ICC/IF Image

Influenza A Virus PA antibody detects PA protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PA protein stained by Influenza A Virus PA antibody (GTX125932) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125932 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX125932) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen J et al. Virol Sin 2023; 38 (1) : 128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
G?nl F et al. Nat Commun 2023; 14 (1) : 787 The ubiquitination landscape of the influenza A virus polymerase.
Chen Y et al. Influenza Other Respir Viruses 2023; 17 (3) : e13112 Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza.
Yang ML et al. J Biomed Sci 2023; 30 (1) : 14 Upregulation of galectin-3 in influenza A virus infection promotes viral RNA synthesis through its association with viral PA protein.
Zheng H et al. J Virol 2022; 96 (12) : e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Show More +
Submit a Reference

REVIEW

Influenza A virus PA protein antibody Cat. No. GTX125932

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015.PMID: 26030097
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28169297
Submit a Review
Application Reference
Chen J et al. Virol Sin 2023; 38 (1):128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
G?nl F et al. Nat Commun 2023; 14 (1):787 The ubiquitination landscape of the influenza A virus polymerase.
Chen Y et al. Influenza Other Respir Viruses 2023; 17 (3):e13112 Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza.
Yang ML et al. J Biomed Sci 2023; 30 (1):14 Upregulation of galectin-3 in influenza A virus infection promotes viral RNA synthesis through its association with viral PA protein.
Zheng H et al. J Virol 2022; 96 (12):e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Jianyuan Zhao et al. Nat Commun 2022; 13 (1):2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.
Application : WB
Xiaoman Liu et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Hatakeyama D et al. FEBS J 2021; (Epub) Acetylation of the influenza A virus polymerase subunit PA in the N-terminal domain positively regulates its endonuclease activity.
Application : WB
Van den Hoecke S et al. mBio 2021; 12 (4):e0074521 In Vivo Therapy with M2e-Specific IgG Selects for an Influenza A Virus Mutant with Delayed Matrix Protein 2 Expression.
Application : WB
Slaine PD et al. J Virol 2021; Thiopurines activate an antiviral unfolded protein response that blocks influenza A virus glycoprotein accumulation.
Application : WB
Walker AP et al. bioRxiv 2020; (Epub) Enisamium is a small molecule inhibitor of the influenza A virus and SARS-CoV-2 RNA polymerases.
Rigby RE et al. Sci Rep 2019; 9 (1):7216 PA-X antagonises MAVS-dependent accumulation of early type I interferon messenger RNAs during influenza A virus infection.
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Sethy B et al. J Med Chem 2019; 62 (5):2390-2403 Design, Synthesis, and Biological Evaluation of Itaconic Acid Derivatives as Potential Anti-Influenza Agents.
Application : WB
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Kupke SY et al. J Virol 2018; (Epub) A novel type of influenza A virus-derived defective interfering particle with nucleotide substitutions in its genome.
Application : WB
Wang L et al. Nat Commun 2017; 8:13876 Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction.
Application : WB
Reactivity : Influenza A virus
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Application : WB
Xu G et al. Virology 2017; 508:45-53 PA-X protein contributes to virulence of triple-reassortant H1N2 influenza virus by suppressing early immune responses in swine.
Application : WB
Sadewasser A et al. Mol Cell Proteomics 2017; (Epub) Quantitative proteomic approach identifies Vpr binding protein as novel host factor supporting influenza A virus infections in human cells.
Application : WB
Reactivity : Influenza A virus
Hu J et al. Med Microbiol Immunol 2016; (Epub) iTRAQ-based quantitative proteomics reveals important host factors involved in the high pathogenicity of the H5N1 avian influenza virus in mice.
Application : ICC/IF
Hu J et al. Med Microbiol Immunol 2016; 205 (4):381-95 PA-X-associated early alleviation of the acute lung injury contributes to the attenuation of a highly pathogenic H5N1 avian influenza virus in mice.
Application : ICC/IF
Sun H et al. Sci Rep 2015; 5:10130 The infection of turkeys and chickens by reassortants derived from pandemic H1N1 2009 and avian H9N2 influenza viruses.
Application : WB
Gao H et al. J Gen Virol 2015; 96 (9):2587-94 PA-X is a virulence factor in avian H9N2 influenza virus.
Application : WB
Reactivity : Influenza A virus
Wu NC et al. PLoS Genet 2015; 11 (7):e1005310 Functional Constraint Profiling of a Viral Protein Reveals Discordance of Evolutionary Conservation and Functionality.
Application : WB
Li W et al. J Virol 2014; (Epub) Interactions between the Influenza A Virus RNA Polymerase Components and Retinoic Acid-Inducible Gene I.
Application : WB
Reactivity : Influenza A virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169