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Influenza A virus PA protein antibody

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932
Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

Cat No. GTX125932

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus PA (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Polymerase acidic protein , Influenza A Virus PA

Research Area

DATA IMAGES

Anti-Influenza A virus PA protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125932

GTX125932 ICC/IF Image

Influenza A Virus PA antibody detects PA protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PA protein stained by Influenza A Virus PA antibody (GTX125932) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125932 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PA protein antibody used in Western Blot (WB). GTX125932

GTX125932 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (GTX125932) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169