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Influenza A virus PB1 protein antibody

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

Cat. No. GTX125923

Reference 49
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus PB1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

DF1 (WSN virus treatment for 10hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2753122

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 1 , Influenza A Virus PB1

Research Area

DATA IMAGES

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Influenza A Virus PB1 antibody detects protein by western blot analysis.
A. 5 μg DF1 whole cell lysate/extract (untreated)
B. 5 μg DF1 whole cell lysate/extract (WSN virus treatment for 10hr)
7.5% SDS-PAGE
Influenza A Virus PB1 antibody (GTX125923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

GTX125923 ICC/IF Image

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB1 protein stained by Influenza A Virus PB1 antibody (GTX125923) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (GTX125923) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen J et al. Virol Sin 2023; 38 (1) : 128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
G?nl F et al. Nat Commun 2023; 14 (1) : 787 The ubiquitination landscape of the influenza A virus polymerase.
Zheng H et al. J Virol 2022; 96 (12) : e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34) : e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
French H et al. Sci Adv 2022; 8 (36) : eabp8655 Transient RNA structures cause aberrant influenza virus replication and innate immune activation.
Show More +
Submit a Reference

REVIEW

Influenza A virus PB1 protein antibody Cat. No. GTX125923

Rating
( Average 5 based on 8 users reviews)
Application
Western Blot(WB)
( Average 5 based on 8 users reviews)
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 19-Apr-2022
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : MDCK cells with/without Influenza A virus (A/Puerto Rico/8/1934) infection
Amount used : 15μg
Blocking : 5% skim milk in 0.1% TBS-T, 20°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28842649
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019. PMID: 31068418
Show More +
Submit a Review
Application Reference
Chen J et al. Virol Sin 2023; 38 (1):128-141 Nonmuscle myosin IIA promotes the internalization of influenza A virus and regulates viral polymerase activity through interacting with nucleoprotein in human pulmonary cells.
G?nl F et al. Nat Commun 2023; 14 (1):787 The ubiquitination landscape of the influenza A virus polymerase.
Zheng H et al. J Virol 2022; 96 (12):e0049422 G Protein Subunit β1 Facilitates Influenza A Virus Replication by Promoting the Nuclear Import of PB2.
Li Q et al. Adv Sci (Weinh) 2022; 9 (34):e2203088 SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus.
French H et al. Sci Adv 2022; 8 (36):eabp8655 Transient RNA structures cause aberrant influenza virus replication and innate immune activation.
Jianyuan Zhao et al. Nat Commun 2022; 13 (1):2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.
Application : WB
Sonja Fernbach et al. Cell Rep 2022; 38 (12):110549 Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.
Application : WB
Ma C et al. PLoS Pathog 2022; 18 (2):e1010295 p21 restricts influenza A virus by perturbing the viral polymerase complex and upregulating type I interferon signaling.
Application : WB
Junping Li et al. PLoS Pathog 2021; 17 (2):e1009336 Viral RNA-binding ability conferred by SUMOylation at PB1 K612 of influenza A virus is essential for viral pathogenesis and transmission.
Application : WB, ICC/IF
Amol Patil et al. J Virol 2021; 95 (6) Phosphorylation of Influenza A Virus NS1 at Serine 205 Mediates Its Viral Polymerase-Enhancing Function.
Application : WB
Vanessa Gerlt et al. Int J Mol Sci 2021; 22 (20) Cellular Protein Phosphatase 2A Regulates Cell Survival Mechanisms in Influenza A Virus Infection.
Application : WB
Froggatt HM et al. PLoS Pathog 2021; 17 (9):e1009951 Influenza A virus segments five and six can harbor artificial introns allowing expanded coding capacity.
Application : WB
Liu X et al. Nat Commun 2021; 12 (1):4427 MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
Application : WB
Deinhardt-Emmer S et al. Biomolecules 2021; 11 (6) Inhibition of Phosphatidylinositol 3-Kinase by Pictilisib Blocks Influenza Virus Propagation in Cells and in Lungs of Infected Mice.
Application : WB
Gong W et al. J Virol 2021; Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly.
Application : WB
Sarto J.D. et al. bioRxiv 2021; (Epub) Phosphorylation of JIP4 at S730 presents anti-viral properties against influenza A virus infection.
Zhu Y et al. Sci Adv 2020; 6 (25):eaaz5764 Human TRA2A determines influenza A virus host adaptation by regulating viral mRNA splicing.
Sun N et al. Cell Rep 2020; 33 (5):108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.
Application : WB
Gao Q et al. J Virol 2020; 95 (2) Eukaryotic Translation Elongation Factor 1 Delta Inhibits the Nuclear Import of the Nucleoprotein and PA-PB1 Heterodimer of Influenza A Virus.
Application : WB
Carrique L et al. Nature 2020; 587 (7835):638-643 Host ANP32A mediates the assembly of the influenza virus replicase.
Application : WB
Chida J et al. PLoS Pathog 2020; 16 (8):e1008823 Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.
Application : WB
Zhang X et al. Front Microbiol 2020; 11:538355 Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
Application : WB
Walker AP et al. J Virol 2020; 94 (13) Mutation of an Influenza Virus Polymerase 3' RNA Promoter Binding Site Inhibits Transcription Elongation.
Application : WB
Hu A et al. Front Microbiol 2020; 11:178 Anthralin Suppresses the Proliferation of Influenza Virus by Inhibiting the Cap-Binding and Endonuclease Activity of Viral RNA Polymerase.
Application : WB
Zhang T et al. Cell 2020; 180 (6):1115-1129 Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis.
Application : WB
Wang R et al. J Virol 2019; 93 (4) Autophagy Promotes Replication of Influenza A Virus In Vitro.
Chao TL et al. Front Microbiol 2019; 10:2942 Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells.
Application : WB
Russell AB et al. J Virol 2019; 93 (14) Single-Cell Virus Sequencing of Influenza Infections That Trigger Innate Immunity.
Hao X et al. Transbound Emerg Dis 2019; (Epub) The PB2 and M genes are critical for the superiority of genotype S H9N2 virus to genotype H in optimizing viral fitness of H5Nx and H7N9 avian influenza viruses in mice.
Application : WB
Choi JG et al. Sci Rep 2019; 9 (1):12132 Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition.
Application : WB
Arai Y et al. PLoS Pathog 2019; 15 (7):e1007919 PB2 mutations arising during H9N2 influenza evolution in the Middle East confer enhanced replication and growth in mammals.
Application : WB
Hao X et al. Virology 2019; 535:218-226 The PB2 and M genes of genotype S H9N2 virus contribute to the enhanced fitness of H5Nx and H7N9 avian influenza viruses in chickens.
Application : WB
Zheng W et al. Cell Rep 2019; 27 (6):1875-1885.e5 Naproxen Exhibits Broad Anti-influenza Virus Activity in Mice by Impeding Viral Nucleoprotein Nuclear Export.
Application : ICC/IF
Zhang J et al. Eur J Pharm Sci 2019; 133:86-94 Identification of NMS-873, an allosteric and specific p97 inhibitor, as a broad antiviral against both influenza A and B viruses.
Application : WB
Mohl G et al. Antiviral Res 2019; 164:81-90 Novel influenza inhibitors designed to target PB1 interactions with host importin RanBP5.
Li J et al. Biochem J 2019; 476 (3):467-481 MOV10 sequesters the RNP of influenza A virus in the cytoplasm and is antagonized by viral NS1 protein.
Application : WB
Nilsson-Payant BE et al. J Virol 2018; 92 (16) The Surface-Exposed PA 51-72-Loop of the Influenza A Virus Polymerase Is Required for Viral Genome Replication.
Lin HT et al. Sci Rep 2018; 8 (1):17242 Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
Application : WB
Yang C et al. J Virol 2018; (Epub) The nucleolar protein LYAR facilitates ribonucleoprotein assembly of influenza A virus.
Application : WB, ICC/IF
Wang J et al. Viruses 2018; 10 (6) Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus.
Application : WB
Chen SC et al. J Virol 2017; 91 (20) Zinc Finger-Containing Cellular Transcription Corepressor ZBTB25 Promotes Influenza Virus RNA Transcription and Is a Target for Zinc Ejector Drugs.
Oymans J et al. J Virol 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Oymans J et al. bioRxiv 2017; (Epub) A mechanism for prime-realignment during influenza A virus replication.
Application : WB
Reactivity : Influenza A virus
Watanabe K et al. Sci Rep 2017; 7 (1):9500 Structure-based drug discovery for combating influenza virus by targeting the PA-PB1 interaction.
Application : WB
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Application : WB
Du Y et al. MBio 2016; 7 (6) Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis.
Application : ICC/IF
Arai Y et al. PLoS Pathog 2016; 12 (4):e1005583 Novel Polymerase Gene Mutations for Human Adaptation in Clinical Isolates of Avian H5N1 Influenza Viruses.
Application : WB
Su WC et al. J Virol 2015; 89 (22):11245-55 A Nucleolar Protein, Ribosomal RNA Processing 1 Homolog B (RRP1B), Enhances the Recruitment of Cellular mRNA in Influenza Virus Transcription.
Application : WB
Reactivity : Influenza A virus
Hsu SF et al. J Virol 2015; 89 (7):3671-82 A Host Susceptibility Gene, DR1, Facilitates Influenza A Virus Replication by Suppressing Host Innate Immunity and Enhancing Viral RNA Replication.
Application : WB
Reactivity : Influenza A virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169