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Influenza A virus PB1 protein antibody

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

Cat No. GTX125923

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

WSN virus infected DF1 10hr
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (GTX125923) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Influenza A Virus PB1 antibody detects protein by western blot analysis.
A. 5 µg DF1 whole cell lysate/extract (untreated)
B. 5 µg DF1 whole cell lysate/extract (WSN virus treatment for 10hr)
7.5% SDS-PAGE
Influenza A Virus PB1 antibody (GTX125923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

GTX125923 ICC/IF Image

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB1 protein stained by Influenza A Virus PB1 antibody (GTX125923) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169