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Influenza A virus PB1 protein antibody

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923
Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

Cat No. GTX125923

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

WSN virus infected DF1 10hr

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2753122

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 1 , Influenza A Virus PB1

Research Area

DATA IMAGES

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (GTX125923) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Western Blot (WB). GTX125923

GTX125923 WB Image

Influenza A Virus PB1 antibody detects protein by western blot analysis.
A. 5 μg DF1 whole cell lysate/extract (untreated)
B. 5 μg DF1 whole cell lysate/extract (WSN virus treatment for 10hr)
7.5% SDS-PAGE
Influenza A Virus PB1 antibody (GTX125923) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125923

GTX125923 ICC/IF Image

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB1 protein stained by Influenza A Virus PB1 antibody (GTX125923) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

REFERENCE

REVIEW

Influenza A virus PB1 protein antibody Cat No. GTX125923

Rating ( Average 5 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 7 users reviews)
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : H1N1
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, 1:2000, 25°C, 1Hr
Lane Description : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28842649
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019.PMID: 31068418
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf