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Influenza A virus PB2 protein antibody

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125925
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125925

Cat No. GTX125925

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Influenza A virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to N-terminal region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125925

GTX125925 ICC/IF Image

Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125925) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37°C, 30 min.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125925

GTX125925 WB Image

Sample (50 µg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
7.5% SDS PAGE
GTX125925 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf