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Influenza A virus PB2 protein antibody

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125925
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125925

Cat. No. GTX125925

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Influenza A virus
Package
100 μl ($399),
25 μl ($169)
See all Influenza A virus PB2 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

WSN-infected DF-1 cell

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170601

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 2 , Influenza A Virus PB2

Research Area

DATA IMAGES

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125925

GTX125925 ICC/IF Image

Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125925) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125925

GTX125925 WB Image

Sample (50 μg of whole cell lysate)
A: mock DF-1 cell lysates
B: WSN-infected DF-1 cell lysates
7.5% SDS PAGE
GTX125925 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chen Y et al. Influenza Other Respir Viruses 2023; 17 (3) : e13112 Antiviral effect and mechanism of Phillyrin and its reformulated FS21 against influenza.
Jianyuan Zhao et al. Nat Commun 2022; 13 (1) : 2079 An anti-influenza A virus microbial metabolite acts by degrading viral endonuclease PA.
Application : WB
Masami Shiimori et al. iScience 2021; 24 (6) : 102660 Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation.
Application : WB
Sun N et al. Cell Rep 2020; 33 (5) : 108342 Type-IInterferon-Inducible SERTAD3 Inhibits Influenza A Virus Replication by Blocking the Assembly of Viral RNA Polymerase Complex.
Application : WB
Chen KY et al. PLoS Pathog 2019; 15 (10) : e1008034 Influenza virus polymerase subunits co-evolve to ensure proper levels of dimerization of the heterotrimer.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169