GeneTex
  • Country / Location Selection

United States (US)

Influenza A virus PB2 protein antibody

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125926

Cat. No. GTX125926

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

WSN virus infected DF1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162999

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 2 , Influenza A Virus PB2

Research Area

DATA IMAGES

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (GTX125926) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

0.5 μg Influenza A viral lysate (0.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (GTX125926) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125926 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125926

GTX125926 ICC/IF Image

Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125926) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

REFERENCE

REVIEW

Influenza A virus PB2 protein antibody Cat. No. GTX125926

Rating ( Average 4.9 based on 18 users reviews)
Application
Western Blot(WB) ( Average 4.9 based on 18 users reviews)
Date : Chen-I Hsu submitted on 16-Sep-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : A549 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX213110-01, Undiluted, 25°C, 1Hr
Lane Description :
Left : A549 cells were transfected with pcDNA3-PB2 (WSN) using Lipofectamine3000. The cell was harvested at 24h post-transfection.
Right : A549 cells were infected with Influenza A (WSN) (MOI=0.1) and harvested at 6, 24, 48 hours postinfection.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017.PMID: 28169297
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 29118119
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2018.PMID: 30209172
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Elife in 2019. PMID: 31159925
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Elife in 2019. PMID: 31159925
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Elife in 2019. PMID: 31159925
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Influenza A virus
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019. PMID: 31217244
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019. PMID: 31217244
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019. PMID: 31217244
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019. PMID: 31217244
Date : Anonymous submitted on 07-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected 293T cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Date : Anonymous submitted on
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019.PMID: 31217244
Date : Anonymous submitted on
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019.PMID: 31217244
Date : Anonymous submitted on
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal J Virol in 2019.PMID: 31217244
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169