GeneTex
  • Country / Location Selection

United States (US)

Influenza A virus PB2 protein antibody

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926
Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

Cat No. GTX125926

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Influenza A virus, Influenza A virus ( H1N1)
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

WSN virus infected DF1 , H1N1-infected H1299

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to C-terminal region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Polymerase basic protein 2 , Influenza A Virus PB2

Research Area

DATA IMAGES

Anti-Influenza A virus PB2 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125926

GTX125926 ICC/IF Image

Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis.
Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: PB2 protein stained by Influenza A Virus PB2 antibody (GTX125926) diluted at 1:500.
Blue: DAPI staining.
Yellow: WGA life stained at 37ºC, 30 min.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Sample (5 μg of whole cell lysate)
A: DF1
B: WSN virus infected DF1 10 hr
7.5% SDS PAGE
GTX125926 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

Non-infected (–) and infected (+) H1299 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (GTX125926) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The WB analysis of Influenza A virus PB2 protein antibody was published by Long JS and colleagues in the journal Elife in 2019 . PMID: 31159925

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The WB analysis of Influenza A virus PB2 protein antibody was published by Long JS and colleagues in the journal Elife in 2019 . PMID: 31159925

Anti-Influenza A virus PB2 protein antibody used in Western Blot (WB). GTX125926

GTX125926 WB Image

The WB analysis of Influenza A virus PB2 protein antibody was published by Long JS and colleagues in the journal Elife in 2019 . PMID: 31159925

REFERENCE

REVIEW

Influenza A virus PB2 protein antibody Cat No. GTX125926

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Nov-2014
Score :
Application Tested : WB
Sample Species : Influenza A virus
Sample : Influenza virus-infected 293T cells
Amount used : 20μg
Blocking : 5% skim milk, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169