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Influenza B virus Hemagglutinin (HA) antibody [10B8]

Anti-Influenza B virus Hemagglutinin (HA) antibody [10B8] used in Western Blot (WB). GTX00860
Anti-Influenza B virus Hemagglutinin (HA) antibody [10B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00860

Cat. No. GTX00860

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10B8

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ELISA, Hemagglutinin inhibition, Neutralizing/Inhibition

Reactivity

 Influenza B virus
Package
100 μg ($399)
See all Influenza B virus Hemagglutinin (HA) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IP 1:200
ELISA Assay dependent
Hemagglutinin inhibition Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody reacted with HA protein of all Influenza B virus isolates belonging to Victoria group tested (73 strains) and none of clinical 27 isolates belonging to Yamagata group as examined by PAP staining. It also reacts with Victoria group vaccine strains; Shangdong/7/1997, Malasia/2506/2004. However, note that HA changes during passages and may change reactivity to this antibody. By western blotting, reactivity with B/Malasia/2506/2004 and B/Massachusetts/2/2012 was tested positive.
We do not recommend use of this product for Influenza A virus samples.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Human Influenza B Virus strain Nagasaki/1/87, one of the strains of B/Victoria group

Purification

Purified IgG
From culture supernant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Influenza B virus Hemagglutinin (HA) antibody [10B8] used in Western Blot (WB). GTX00860

GTX00860 WB Image

WB analysis of MDCK cells infected with various Influenza B virus strains using GTX00860 Influenza B Virus Hemagglutinin (HA) antibody [10B8]. This antibody recognizes an epitope on HA2 region.
Lane 1 : B/Mie/1/1993
Lane 2 : B/Johannes Burg/5/1999
Lane 3 : B/Florida/4/2006
Lane 4 : B/Lee/1940
Lane 5 : B/Florida/4/2006
Lane 6 : Shandong/7/97
Lane 7 : B/Malasia/2506/2004
Lane 8 : B/Massachusetts/2/2012
Dilution : 1:500

Anti-Influenza B virus Hemagglutinin (HA) antibody [10B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00860

GTX00860 ICC/IF Image

ICC/IF analysis of MDCK cells were infected with Influenza B virus (B/Malasia/2506/2004, B/Florida/4/2006) using GTX00860 Influenza B Virus Hemagglutinin (HA) antibody [10B8].
Fixation : 4% PFA in PBS
Permeabilization : 0.1% Triton X-100 in PBS

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399