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Influenza B virus Nucleoprotein antibody [GT371]

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in Western Blot (WB). GTX629882
Anti-Influenza B virus Nucleoprotein antibody [GT371] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629882
Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882
Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882
Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882
Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882
Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882

Cat No. GTX629882

Host

Mouse

Clonality

Monoclonal

Clone Name

GT371

Isotype

IgG2a

Application

WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

Influenza B virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX629882, Detection: GTX128539/GTX128538 or Capture: GTX128539, Detection: GTX629882

Not tested in other applications.

Positive Control

Influenza B (Taiwan 70555) infected MDCK

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888172

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Research Area

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in Western Blot (WB). GTX629882

GTX629882 WB Image

Influenza B Virus NP antibody [GT371] detects Influenza B Virus NP protein by Western blot analysis.
A. 1 μg MDCK whole cell lysate/extract
B. 1 μg whole cell lysate/extract of Influenza B virus (Taiwan 70555) infected MDCK cells
7.5 % SDS-PAGE
Influenza B Virus NP antibody [GT371] (GTX629882) dilution: 1:5000

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629882

GTX629882 ICC/IF Image

Influenza B Virus NP antibody [GT371] detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: Influenza B Virus infected MDCK cells were fixed in paraformaldehyde.
Green: Influenza B Virus NP protein stained byInfluenza B Virus NP antibody [GT371] (GTX629882) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882

GTX629882 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882

GTX629882 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, Flag tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882

GTX629882 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882

GTX629882 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody (GTX128539) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [GT371] used in ELISA (ELISA). GTX629882

GTX629882 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128539) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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SDS
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