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Influenza B virus Nucleoprotein antibody [HL1068]

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Lateral Flow (Lateral Flow). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Western Blot (WB). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Western Blot (WB). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Lateral Flow (Lateral Flow). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099
Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

Cat No. GTX636099

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1068

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Influenza B virus
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636194 / GTX636100, Detection : GTX636099 or Capture : GTX636099, Detection : GTX636194 / GTX636100. Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194 / GTX636100. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909945

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Lateral Flow (Lateral Flow). GTX636099

GTX636099 Lateral Flow Image

Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza B virus nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) (50 ng), and probing with the specified influenza B virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Western Blot (WB). GTX636099

GTX636099 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 18.81 pM

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL).

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL).

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Western Blot (WB). GTX636099

GTX636099 WB Image

Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL)

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636099

GTX636099 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza B virus Nucleoprotein antibody (GTX636099).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza B virus Nucleoprotein antibody (GTX636099) diluted at 1:100.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636099

GTX636099 ICC/IF Image

Influenza B virus Nucleoprotein antibody (GTX636099) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX636099) diluted at 1:2000.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in Lateral Flow (Lateral Flow). GTX636099

GTX636099 Lateral Flow Image

Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL)

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1068] used in ELISA (ELISA). GTX636099

GTX636099 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

REFERENCE

There are currently no references for Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099). Be the first to share your publications with this product.

REVIEW

Influenza B virus Nucleoprotein antibody [HL1068] Cat No. GTX636099

Rating ( Average 5 based on 2 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : Lateral Flow
Figure Note : Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
Date : Anonymous submitted on 03-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : Influenza B virus
Sample : Influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
Figure Note : Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Package List Price ($)
$ 379
$ 169
Custom Conjugations