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Influenza B virus Nucleoprotein antibody [HL1069]

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Lateral Flow (Lateral Flow). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Western Blot (WB). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Western Blot (WB). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Lateral Flow (Lateral Flow). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100
Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

Cat No. GTX636100

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1069

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

Influenza B virus
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636099 / GTX636194, Detection : GTX636100 or Capture: GTX636100, Dectection: GTX636099 / GTX636194. Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099 / GTX636194. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Lateral Flow (Lateral Flow). GTX636100

GTX636100 Lateral Flow Image

Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636100

GTX636100 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza B virus Nucleoprotein antibody (GTX636100).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:100.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636100

GTX636100 ICC/IF Image

Influenza B virus Nucleoprotein antibody (GTX636100) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:2000.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Western Blot (WB). GTX636100

GTX636100 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL).

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Western Blot (WB). GTX636100

GTX636100 WB Image

Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL).

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in Lateral Flow (Lateral Flow). GTX636100

GTX636100 Lateral Flow Image

Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 8.72 pM

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL)

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL)

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza B virus Nucleoprotein antibody [HL1069] used in ELISA (ELISA). GTX636100

GTX636100 ELISA Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza B virus nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) (50 ng), and probing with the specified influenza B virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

REFERENCE

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REVIEW

Influenza B virus Nucleoprotein antibody [HL1069] Cat No. GTX636100

Rating ( Average 5 based on 2 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : Lateral Flow
Figure Note : Detection of recombinant influenza B virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
Detection: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
Date : Anonymous submitted on 03-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : Influenza B virus
Sample : Influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
Figure Note : Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)