Influenza B virus Nucleoprotein antibody

Cat. No. GTX128538

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA, Sandwich ELISA
Reactivity	Influenza B virus
Package	100 μl ($399), 25 μl ($169)

See all Influenza B virus Nucleoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:2000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX629882 / GTX629883, Detection : GTX128538

Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

Influenza B virus (Taiwan B70555) infected medium

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885796

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza B Virus Nucleoprotein , Influenza B Virus NP

Research Area

DATA IMAGES

GTX128538 WB Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by Western blot analysis.
A. 5μl non-infected medium
B. 5μl Influenza B virus (Taiwan B70555) infected medium
7.5 % SDS-PAGE
Influenza B Virus NP antibody (GTX128538) dilution: 1:10000

GTX128538 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: Influenza B Virus infected MDCK cells were fixed in paraformaldehyde.
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128538) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX128538 ICC/IF Image

Influenza B Virus NP antibody detects Influenza B Virus NP protein by immunofluorescent analysis.
Sample: MDCK cells infected with Influenza B virus (Taiwan B70555).
Green: Influenza B Virus NP protein stained by Influenza B Virus NP antibody (GTX128538) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128538 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody (GTX128538) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza B virus (B/Sydney/3/2004) nucleoprotein transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, Flag tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT371] (GTX629882) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX128538 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza B virus NP (nucleoprotein) protein, Flag tag (GTX135867-pro) using Influenza B virus Nucleoprotein antibody [GT382] (GTX629883) as capture antibody at concentration of 5 μg/mL and Influenza B virus Nucleoprotein antibody (GTX128538) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

Application Reference

Gravel C et al. iScience 2021; 24 (11) : 103328 Synthetic vaccine affords full protection to mice against lethal challenge of influenza B virus of both genetic lineages.

Application : ELISA

Zhang Z et al. PLoS Pathog 2020; 16 (10) : e1008989 Selective usage of ANP32 proteins by influenza B virus polymerase: Implications in determination of host range.

Application : WB

Jang Y et al. Antiviral Res 2016; 134 : 77-88 Antiviral activity of KR-23502 targeting nuclear export of influenza B virus ribonucleoproteins.

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REVIEW

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Application Reference

Gravel C et al. iScience 2021; 24 (11):103328 Synthetic vaccine affords full protection to mice against lethal challenge of influenza B virus of both genetic lineages.

Application : ELISA

Zhang Z et al. PLoS Pathog 2020; 16 (10):e1008989 Selective usage of ANP32 proteins by influenza B virus polymerase: Implications in determination of host range.

Application : WB

Jang Y et al. Antiviral Res 2016; 134:77-88 Antiviral activity of KR-23502 targeting nuclear export of influenza B virus ribonucleoproteins.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Influenza B virus Nucleoprotein antibody

Cat. No. GTX128538

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Research Area

DATA IMAGES

GTX128538 WB Image

GTX128538 ICC/IF Image

GTX128538 ICC/IF Image

GTX128538 WB Image

GTX128538 ELISA Image

GTX128538 ELISA Image

GTX128538 ELISA Image

GTX128538 ELISA Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.